方法乳腺癌细胞MCF-7转染miR-34a mimics及miR-34a NC,RT-PCR法检测细胞中miR-34a表达,MTT,克隆形成实验,Hoechst染色及流式细胞术分别检测miR-34a mimics对乳腺癌MCF-7细胞活力、克隆能力、凋亡情况及细胞周期的影响;通过双荧光素酶报告基因实验,western blot及RT-PCR检测Notch1是否是miR-34a的下游靶基因。结果与...
34a(miR-34amimics),瞬时转染膀胱癌T24细胞,MTS法检 测miR-34a对T24细胞增殖的影响.结果:在人膀胱癌组织中发现miR-34a较其相应周围非瘤组织明显低表达.在42例膀胱癌组织 中,26(61.9%)例miR-34a显著低表达.miR-34a的低表达与肿瘤大小和恶性程度显著相关,肿瘤越大,恶性程度越高,miR-34a的表 达量越低.转染miR...
mimics上调miR一34a的表达并检测其细胞活力;在正常细胞HEK293中转染miR一34ainhibitors下调miR一34a的表达水平并 检测其细胞凋亡率。结果 照前辐射敏感性高的细胞miR一34a表达水平高,辐射敏感性低的细胞miR一34a表达水平低;照后 辐射敏感性高的细胞miR一34a表达上调幅度远高于辐射敏感性低的细胞。上调miR一34a表达水平...
Con control, M the miR-34a mimics, I the miR-34a inhibitors.*p < 0.05. Full size image To determine the effects of miR-34a in pancreatic cancer, we transfected the miR-34a mimics or inhibitors into pancreatic cancer cell lines. Subsequently, cell migration, invasion and apoptosis assays...
In this first-in-human, Phase 1 study of a microRNA-based cancer therapy, the recommended Phase 2 dose (RP2D) of MRX34, a liposomal mimic of microRNA-34a (miR-34a), was determined and evaluated in patients with advanced solid tumours. Adults with various
adversely affect age- and myocardial infarction-associated viability and senescence of cardiomyocytes110and processes associated with pulmonary fibrosis.111Investigation of a particular miR-34a ‘targetome’ in different cell types may justify appropriately targeted usage of miR-34a mimics in cancer therapy....
and MIR34a levels in serum have been proposed as diagnostic biomarker for PDAC30,32,33,34. Additionally,Mir34ahas a great therapeutic potential35, and it has already been tested in pre-clinical studies, whereMir34amimics (in combination with PLK1 siRNA) was delivered using an amphiphilic nano-...
经TargetScan和miRDB数据库检索及预测,Sirt1与DN病理损伤有关,它可与miR-141结合(图3A)。经双荧光素酶报告基因分析,在Sirt1-WT-3’-UTR转染组,转染miR-141 mimics后,荧光素酶的相对活性较NC组显著降低,而转染miR-141 inhibitor...
摘要: 目的 探讨miR-34a对人成骨肉瘤MG-63细胞增殖的影响.方法 运用miR-34a的mimics或inhibitors分别转染人成骨肉瘤MG-63细胞,分别为实验组,脂质体2000为对照组,采用甲基噻唑基四唑(MTr)法检测细胞增殖情况.结果 对照组吸光度值在24h、48 h和72 h分别为(0.33±0.02)、... 查看全部>> ...
We36,37and others38have reported that overexpression of transforming growth factor (TGF)-β1 in the developing lung mimics the BPD pulmonary phenotype. To determine whether the effects of miR-34a are limited to the hyperoxia-induced BPD model or could be dependent on other injury mediators, we...