为了确定在OA中 miR-326 水平的作用,我们将 miR-326 mimics 转化到OA小鼠模型中(图2A)。结果显示,与对照组相比,BMSC来源的外泌体纳米囊泡和miR-326对软骨层的损伤程度有一定的改善。这些结果表明,外泌体miR-326具有缓解OA的能力。 外泌体miR-326抑制了软骨细胞焦亡 近年来的研究表明,细胞焦亡在软骨疾病的发展...
MiR-326 mimics robustly decreased the collagen I and fibronectin levels and inhibited cell proliferation of TGF-β1-treated ASMCs. Luciferase assay investigated that tumor necrosis factor superfamily member 14 (TNFSF14) was a direct target of miR-326. The expression of TNFSF14 was ne...
To restore physiological levels of a miRNA, mimics or synthetic double-stranded RNA can be employed 93, 94, since they can bind to the RISC complex, achieving suppression of target mRNAs. To explore and validate the effect of miR-326 in pre-clinical models, mimic, inhibitory sequences and ...
To further validate the function of the five putative miRNA candidates, the cell viability of HCT116 cells after transfection of these miRNA mimics was assessed. The results indicated that overexpression of miR-326 and miR-7706 significantly reduced the cell viability of HCT116 cells, while other ...
ABCC1 mRNA degradation was accelerated dramatically in cells transfected with miR-133a or miR-326 mimics using qRT-PCR, Furthermore, western blot analysis indicated that ABCC1 protein expression was significantly down-regulated in hepatocellular carcinoma cells line HepG2 after transfection with miR-...
Moreover, SMAD6 elevation overturned the inhibitory impacts of miR-326 mimics on malignant behaviors of HCC cells. Conclusions: Hsa_circ_0000517 depletion repressed HCC advancement via regulating the miR-326/SMAD6 axis.doi:10.1186/s12935-020-01447-wShuwei He...
目的 探讨lncRNA TUG1对哮喘患儿气道平滑肌细胞凋亡和炎症因子的影响及其分子机制.方法 收集2017年1月～2020年6月我院收治的支气管哮喘患儿和非哮喘患儿气道平滑肌组织;分离培养气道平滑肌细胞(ASMCs),将其分为si-NC组,si-TUG1组,miR-mimics-NC组,miR-326-mimics组,si-NC+miR-inhibitor... 查看全部>>doi:...
微小RNA-326-3p、视网膜祖细胞、分化、视网膜退行性疾病目的探讨微小RNA-326-3p(miR-326-3p)在视网膜祖细胞(RPCs)分化过程中的作用,旨在为干细胞移植治疗视网膜退行性疾病研究提供新的思路.方法将RPCs随机分为对照组(Control组),miR-326-3p过表达组(Mimics组)和miR-326-3p敲降组(Inhibitors组),在分化培养基中培...
Meanwhile, miR‐326 mimics can also inhibit HCC progression, whereas miR‐326 inhibitors exhibited a reverse phenomenon by modulating H19 expression. In addition, a negative association between H19 and miR‐326 was predicted and confirmed. Furthermore, the transcription factor TWIST1 has been ...
To restore physiological levels of a miRNA, mimics or synthetic double-stranded RNA can be employed 93, 94, since they can bind to the RISC complex, achieving suppression of target mRNAs. To explore and validate the effect of miR-326 in pre-clinical models, mimic, inhibitory sequences and ...