为了确定外泌体miR-326表达在敏感患者鉴别耐药病例中可能的预后价值，进行了ROC分析。AUC指数验证了miR-326的预后意义，具有良好的性能（AUC = 0.7755，95%置信区间），灵敏度为57.14%，特异性为100。与药物敏感病例相比，耐多药性患者的外泌体miR-326水平升高。这些结果与之前报告的细胞质miR-326作为儿科B-ALL...
随后,采用qRT-PCR、Western blot和双荧光素酶报告试验确认miR-326靶向EHMT2基因并抑制其翻译过程。拯救试验进一步确认lncFPFSC作为竞争性内源RNA抑制miR-326/EHMT2信号轴的调控作用。 综上,这些结果表明lncFPFSC作为竞争性内源RNA解除...
我们发现,BMSC来源外泌体纳米囊泡中miR-326的水平显著升高,可以通过细胞外小鼠骨髓间充质干细胞(BMSC)治疗骨关节炎软骨损伤。miR-326通过目标靶标HDAC3表达水平的调节,间接抑制了软骨细胞的焦亡,减轻了骨关节炎的软骨损伤。此外,我们还发现,miR-326可以促进BMSC的化学诱导分化为软骨细胞。本研究揭示了BMSC来源外泌体...
为了进一步评估SNHG9基因敲除是否通过上调miR-326来抑制GSC细胞的生长,我们在转染miR-326抑制剂后SNHG9基因敲除的背景下进行了上述细胞功能测定。EDU检测结果显示,miR-326抑制剂的引入有效地抵消了SNHG9基因敲除介导的对GSC-87和GSC-251细...
miR-326 over-expression suppressed cell growth by directly targeting PNO1 To investigate the upstream molecular mechanism of PNO1, potential miRNAs targeting PNO1 were predicted. A total of 1,423 miRNA candidates were predicted to target PNO1 bymiRwalk(Supplemental data TableS3), among of whic...
miR-326 attenuated the ef f ect ofAng II on cardiac hypertrophy in vitro. In addition, miR-326 negatively regulated JAK/STAT and MAPK sig-naling pathways. Mechanistically, miR-326 targeted and inhibited MDK to induce JAK/STAT and MAPK path-ways. Rescue assays certif i ed that miR-326 ...
The upregulation of miR-326 increased radiation sensitivity of the CSCC cell through downregulating SMO and its downstream proteins in the Hedgehog (Hh) signaling pathway. Conclusions miR-326 may predict the treatment response to radiation, and upregulating miR-326 may improve the treatment response ...
We found that the expression of miR-326 was significantly decreased in the serum of osteosarcoma patients and osteosarcoma tumor cells compared to healthy controls (P<0.01). Moreover, a receiver operating characteristic (ROC) curve analysis is indicated that serum miR-326 is a potential diagnostic...
miR-326是一种具有调节免疫功能的微小RNA,能够影响Th17和Treg的分化及功能。研究表明,SOCS1/STAT3/miR-326在调节Th17/Treg平衡中发挥重要作用。 五、新风胶囊合芙蓉膏调节RA患者Th17/Treg的机制 新风胶囊合芙蓉膏在调节RA患者Th17/Treg平衡的过程中,可能通过以下机制发挥作用: 1.调节SOCS1的表达:新风胶囊合芙蓉膏...
Among others, miR-326 has been shown to act as tumour suppressor in various types of tumours and its expression is deregulated in both adult and paediatric brain cancers 20, 21, 22. However, although its role in neurological diseases and non-central nervous system (CNS) solid cancers has ...