All short-read sequencing technologies have a common limitation – the inability to sequence long stretches of DNA. To sequence a large stretch of DNA using NGS, such as a human genome, the strands have to be fragmented and amplified.Bioinformatic programsare then used to assemble these random ...
The present study provides a comprehensive resource of five BMI and DSE reproductive glands. As the dataset contains both long-read and short-read sequencing data, this rich sequencing dataset paves the avenue for quantifying gene or transcript expression, evaluating alternative splicing, delving novel...
Sentieon | 每周文献-Long Read Sequencing(长读长测序)-第七期,研究者通过将PacBio长读长数据进行组装后对基因组进行repeat和gene注释。然后,利用测序或者从公开发表文献中下载的棉花基因组数据进行比对、变异检测、联合基因分型对亚洲栽培棉和非洲亚种进行比较。此过
Iyer, Sara Goodwin, and William Richard McCombie Abstract: “Long-read sequencing technologies have improved the contiguity and, as a result, the quality of genome assemblies by generating reads long enough to span and resolve complex or … more »...
在2016年,首届瓶中基因组(GIAB)-precisionFDA真相挑战赛正式启幕,挑战参赛者从两个GIAB样本的短读长数据中识别出小变体。虽然HG001(也被称为NA12878)的基准测试数据已提前公布,但当时并未公开HG002的基准测试数据,这使得该挑战成为首个盲法种系变异检测挑战。其结果公开后,已被用作评估新变异检测方法的基准。尽管目...
Sequencing data analysis All programs used default parameters unless stated otherwise in the corresponding section. Single-cell short-read sequencing Single-cell analysis was carried out using the Seurat R package (v3.2)8. We filtered cells using the following criteria: (a) feature counts below 500...
A modified Chromium 10x droplet-based protocol that subsamples cells for both short-read and long-read (nanopore) sequencing together with a new computational pipeline (FLAMES) is developed to enable isoform discovery, splicing analysis, and mutation det
Targeted-hybridization short-read sequencing The Genomic DNA Extraction Kit (Vazyme, Nanjing, China) was used to extract genomic DNA according to the instructions of the manufacturer. The DNA concentration was quantified using the Qubit dsDNA HS Assay Kit (Thermo Fisher). 500 ng of DNA was divide...
Long-read technologies are overcoming early limitations in accuracy and throughput, broadening their application domains in genomics. Dedicated analysis tools that take into account the characteristics of long-read data are thus required, but the fast pa
Out of the many pathogenic bacterial species that are known, only a fraction are readily identifiable directly from a complex microbial community using standard next generation DNA sequencing. Long-read sequencing offers the potential to identify a wider