Long‐read sequencing (LRS) has been around for more than a decade, but widespread adoption of the technology has been slow due to the perceived high error rates and high sequencing cost. This is changing due to the recent advancements to produce highly accurate sequences and the reducing ...
PacBio incorporated the NVIDIA A100 Tensor Core GPU into its Revio system to accelerate the speed and accuracy of long‑read sequencing, while minimizing costs.
higher error rate, and higher costs of the instruments have kept them from being more widely adopted. With recent advancements such as the PacBioRevioandVega, and the ONTGridIONandPromethION, the scale and cost of long read sequencing is now comparable base-for-base against ...
Over the past decade, long-read, single-molecule DNA sequencing technologies have emerged as powerful players in genomics. With the ability to generate reads tens to thousands of kilobases in length with an accuracy approaching that of short-read sequencing technologies, these platforms have proven ...
Ranallo-Benavidez T R, Lemmon Z, Soyk S, et al. Optimized sample selection for cost-efficient long-read population sequencing[J]. Genome Research, 2021, 31(5): 910-918.https://www.genome.org/cgi/doi/10.1101/gr.264879.120. 当使用低分辨率方法(如芯片、外显子捕获、短读长WGS等)对大型群体...
To demonstrate the utility of an amplification-free long-read sequencing method to characterize the Fuchs endothelial corneal dystrophy (FECD)-associated intronic TCF4 triplet repeat (CTG18.1). We applied an amplification-free method, utilizing the CRISP
We are not aware of any previously published direct comparisons of hybrid bacterial assemblies generated using long-read sequencing methods, yet the selection of a long-read sequencing approach has important cost, throughput and logistical implications. Currently, the two dominant long-read technologies ...
In recent years, the development of long-read sequencing technologies and concomitant advances in their cost-efficiency and accuracy has brought disruptive change to a variety of important biological applications. Long-read sequencing has largely trivialized the generation of complete and accurate de ...
Short-read sequencing is cost-effective, accurate, and supported by a wide range of analysis tools and pipelines [9]. However, natural nucleic acid polymers span eight orders of magnitude in length, and sequencing them in short amplified fragments complicates the task of reconstructing and counting...
(Table3) in the general population using LRS. We expect that the increasing use of LRS in clinical studies and matched population controls will deepen our knowledge and interpretation of SVs and the decrease in sequencing cost will lead to progressively larger studies, as already seen for some ...