hek293 as a recombinant protein factory three different approaches for protein productionusing RMCE technology (Figure 1C). Using this approach, CapPCV2 gene was inserted into previously identified chromosomal hotspots. The specific produc- tion of the RMCE-derived stable cell line (0.226 ± 0.034 ...
[2] Gray D. Overview of protein expression by mammalian cells. Curr Protoc Protein Sci. 2001 May;Chapter 5(1):Unit5.9. doi: 10.1002/0471140864.ps0509s10. PMID: 18429190; PMCID: PMC7162368. [3] Hacker DL, Balasubramanian S. Recombinant protein production from stable mammalian cell lines and...
[2] Gray D. Overview of protein expression by mammalian cells. Curr Protoc Protein Sci. 2001 May;Chapter 5(1):Unit5.9. doi: 10.1002/0471140864.ps0509s10. PMID: 18429190; PMCID: PMC7162368. [3] Hacker DL, Balasubramanian S. Recombinant protein production from stable mammalian cell lines and...
EMP Genetech provides non-GMP services in recombinant protein production and the development of stable high producer cell lines. Protein purification to customer given purity is also conducted, as well as cell banking. We work with industrial and academical clients. Since 2000 the company has develop...
The study used HEK293 cells to develop a high cell-density perfusion method for recombinant human Erythropoietin protein production.Zika virus-like particles (VLPs): Stable cell lines and continuous perfusion processes as a new potential vaccine manufacturing platform Vaccine The publication reported using...
[2] Dietmair S, Hodson MP, Quek LE, Timmins NE, Gray P, Nielsen LK. A multi-omics analysis of recombinant protein production in Hek293 cells. PloS One 2012; 7(8):e43394. [3] Doig CJ, Zygun DA, Delaney A, Manns BJ. Drotrecogin alfa (activated; Xigris): An effective and co...
The study used HEK293 cells to develop a high cell-density perfusion method for recombinant human Erythropoietin protein production. Zika virus-like particles (VLPs): Stable cell lines and continuous perfusion processes as a new potential vaccine manufacturing platform ...
The study used HEK293 cells to develop a high cell-density perfusion method for recombinant human Erythropoietin protein production. Zika virus-like particles (VLPs): Stable cell lines and continuous perfusion processes as a new potential vaccine manufacturing platform ...
[2] Gray D. Overview of protein expression by mammalian cells. Curr Protoc Protein Sci. 2001 May;Chapter 5(1):Unit5.9. doi: 10.1002/0471140864.ps0509s10. PMID: 18429190; PMCID: PMC7162368. [3] Hacker DL, Balasubramanian S. Recombinant protein production from stable mammalian cell lines and...
Transient gene expression: recombinant protein production with suspension-adapted HEK293-EBNA cells[J]. Biotechnol Bioeng, 2001, 75(2): 197-203. [5] Geisse S, Henke M. Large-scale transient transfection of mammalian cells: a newly emerging attractive option for recombinant protein production[J]...