producer cell lines. Protein purification to customer given purity is also conducted, as well as cell banking. We work with industrial and academical clients. Since 2000 the company has developed several dozen recombinant biomolecules. EMP Genetech aims to support companies in the biotechnology and th...
We have generated more than 30 parallel vectors for expression in bacteria, insect and HEK293 cells with different purification and solubility enhancing tags that we consider to be helpful in our workflow. This list of pCoofys has fundamentally increased our throughput and success rate in protein ...
Expression and Purification of Protein Complexes Suitable for Structural Studies Using Mammalian HEK 293F Cells.doi:10.1002/cpps.44HEK293FcellculturemammaliancellsproteincomplexestransienttransfectionProkaryotic expression systems have been widely used to express proteins for structural studies. Such expression ...
Customized protein purificationProtein purification is a critical step, and selecting the appropriate method is essential to obtain superior protein products. Our expert team can guide you in selecting the most suitable methodology tailored to your specific application needs. Purified proteins can be used...
Magnetic beads and KingFisher Purification Systems enable the automation of immunoprecipitation (IP) and other affinity purification protocols for proteomics analysis by mass spectrometry. Learn more Featured data Enrichment using streptavidin magnetic beads...
c) IMAC purification is performed using Nickel-coated chelating Sepharose. d) A final round of gel filtration is usually sufficient to yield proteins of over 90% purity. The above figure reveals the efficient production of a receptor-ligand complex by co-transfection in HEK293T cells. Cells wer...
PP2A-A protein levels are reduced after GVBD. Thus, we hypothesized that CRL4 activity should be regulated during meiosis. However, it was difficult to determine the CRL4 Ub ligase activity at different stages because the small numbers and sizes of mouse oocytes prevented the purification of end...
Synonyms Coronavirus;2019-nCov Spike protein;2019-nCov-S RBD;2019-nCov S RBD;COVID-19;COVID-19 RBD protein;COVID-19 RBD Purification >95% as determined by SDS-PAGE. Description Recombinant COVID-19 Spike protein (RBD), Biotin is expressed from Insect cells. We can offer labeled antibodies...
Extracellular vesicles (EVs) are small nanometer-sized membrane sacs secreted into biological fluids by all cells. EVs encapsulate proteins, RNAs and metabolites from its origin cell and play important roles in intercellular communication events. Over the past decade, EVs have become a new emerging ...
Interacting proteins were also scrutinized in the Contaminant Repository for Affinity Purification (CRAPome) database (Mellacheruvu et al., 2013) to determine the occurrence of proteins in control experiments to eliminate “sticky proteins” that may non-specifically interact with HuR. The final list ...