Transient gene ex- pression in HEK293 cells: peptone addition posttransfection improves recombinant protein synthesis. Biotechnol Bioeng 2005;90(3):332-44.Pham PL, Perret S, Cass B, Carpentier E, St-Laurent G, Bisson L, Kamen A, Durocher Y: Transient gene expression...
Transient transfection of mammalian cells is used in the biotechnology industry to quickly supply recombinant protein for research and large molecule drug development. Here, we describe a method for high throughput transient transfection of Human Embryonic Kidney 293 (HEK293) cells in 30 mL tubespins ...
After concentrating cells to such high densities, transfection of HEK-293 cells becomes possible in most commonly used media and is not restricted to a specific medium. Furthermore, there is no need to make transfection complexes a priori, a step that prevents inline sterile filtration of the ...
[translate] aregia 方向 [translate] aYou could be the one . 正在翻译,请等待... [translate] ahajdubagos hajdubagos [translate] aSpectral analysis 光谱分析 [translate] aOne day prior to transfection, HEK 293 cells were seeded in p60 一天在transfection之前, HEK 293细胞在p60播种了 [translate] ...
(1999) Analysis of a YAC with human telomeres and oriP from epstein-barr virus in yeast and 293 cells, Nucl, Acids Res 27,18, 3736 CrossRef About this Chapter Title High Throughput Transient Transfections of Hek293Ebna Cells — Devel Opment of an Integrated Expression Purification Process...
Supports transient transfection, growth, and expression of a variety of recombinant proteins in HEK 293 cells. From64.17 USD Configure your product Pack size 5 L 10 L 50 L 500 mL 1000 mL To buy, customize your product. Price includesstandard shipping and handling ...
TransIT®-293 Transfection Reagent – 5 X 1.0 mL TransIT®-293 Transfection Reagent – 10 X 1.0 mL Description Resources Description Unique Formulation– Maximize transfection performance in HEK 293 cells High Efficiency Delivery– Achieve 75-85% transfection efficiency to ensure experimental success ...
Researchers used a cationic polymer to transfect HEK293 cells (grown in suspension). The cationic polymer (both branched and linear) was successful at transfection and is scalable. The resultant proteins were produced at significant levels without the typical, undesired production properties such as me...
Since these questions are important to evaluate the results obtained with HCR, in the present paper we studied the fate of the plasmid pEGFP-N1 after transfection in HEK 293 cells. To this end nuclei isolated from cells transfected with native and trioxsalen crosslinked pEGFP-N1 were digested ...
Optimized for transient transfection in CHO suspension cells and also works for HEK-293 cells Used for large-scale transient protein production with milligrams of protein yield Lipofectamine 2000 CD Transfection Reagent Same performance as Invitrogen Lipofectamine 2000, certified animal-origin free (“CD”...