HEK 293 cells are popular for their ease of growth and transfection (HEK293 Transfection Kit), making them a common cell culture in cancer research. In addition, high transfection efficiency of HEK293 cells produces exogenous proteins or viruses for pharmaceutical and biomedical research purposes. HE...
Transfection efficiency was determined by qRT-PCR.Product Description:Transfection reagent is specially designed for HEK293 cellline, a human epithelial kidney cells. High efficiency delivering both DNA and RNA into HEK293 cells.Transfection Protocol and SDS:Download Altogen Biosystems HEK-293 Transfection...
Based on a previous characterization of the HEK293 cell line upon transient transfection using multiplexed quantitative proteomics, molecular production bottlenecks and metabolic pathways likely to be optimized were identified. In this study, these molecular components and metabolic pathways have been ...
中文名称:HEK293细胞瞬时转染无血清培养基(电穿孔法) 中文同义词:HEK293细胞瞬时转染无血清培养基(电穿孔法) 英文名称:HEK293 cell transient transfection serum-free medium (electroporation) 英文同义词:HEK293 cell transient transfection serum-free medium (electroporation) ...
The Calcium Phosphate Transfection technique is routinely employed to introduce cDNAs into HEK293T, HEK293, CHO and BHK cells, and has been reported to work effectively in more highly differentiated cell lines, including neuronal cell lines. FIVEphoton Biochemicals recommends that the researcher should...
Cell Type:HEK-293 *The parental HEK cell line was genotyped to verify its identity prior to generation of the IP3R cell lines Accession ID:Q14643, Q14571, Q14573, CVCL_HB82, CVCL_HB83, CVCL_HB84, CVCL_HB85, CVCL_HB86, CVCL_HB87, CVCL_HB88 ...
In addition, although blasticidin selection is required for this cell line, it may not be necessary for other protein-expressing HEK cell lines. Figure 2:The evaluation of clumping in suspension 293/hTLR4-HA cells: A: Cells formed large aggregates containing > 50 cells, this culture received ...
Growth in gene therapies and viral vector vaccines has increased the demand for robust HEK293 cell lines. Sartorius offers four high-performing suspension HEK media that are designed for use with the high variability of HEK293 cell lines, the versatility of adeno-associated virus and the complexity...
Yet, most production methods in mammalian cells involve the time- and labor-consuming step of creating stable cell lines. Production methods based on transient gene expression are advantageous in terms of speed and versatility; yet, depending on the transfection protocol, transient transfection faces ...
2.2HEK293 cell culture and transfection Human Embryonic Kidney-293 cells (HEK 293; American Type Culture Collection, Manassas, VA) were grown in Dulbecco’s modified Eagle’s medium (DMEM, Invitrogen) supplemented with 10% fetal bovine serum (FBS). Cells were thawed, maintained in 5% CO2at 37...