and L.R. Castilho, Zika virus-like particles (VLPs): Stable cell lines and continuous perfusion processes as a new potential vaccine manufacturing platform. Vaccine, 2019. 37(47): p. 6970-6977.Schwarz, H., et al., Small-scale bioreactor supports high density HEK293 cell perfusion culture ...
1. 贴壁培养(Adherent culture)：这是最常见的细胞培养方式，使用含有血清的培养基，细胞贴附在容器表面生长。通常使用含有10% FBS的DMEM培养基在T-flasks中培养HEK-293T细胞。2. 悬浮培养(Suspension culture)：这是一种细胞不贴附在容器表面的培养方式，可以获得更高的细胞密度。3. 批次培养(Batch culture)：这...
FAQ Cell Lines Related products NF-κB–SEAP and IRF–Lucia reporter HEK293 cells expressing human TLR9Signaling pathways in HEK-Dual™ hTLR9 cells HEK-Dual™ hTLR9 cells were were engineered from HEK-Dual™ cells, a human embryonic kidney HEK293-derived cell line, to study the Toll-...
Pulix, M., et al., Molecular characterization of HEK293 cells as emerging versatile cell factories. Current Opinion in Biotechnology, 2021.71: p. 18-24. Alvim, R.G., I. Itabaiana Jr, and L.R. Castilho, Zika virus-like particles (VLPs): Stable cell lines and continuous perfusion process...
HEK-Dual™ hTLR8 cells were engineered from HEK-Dual™ cells, a human embryonic kidney HEK293-derived cell line, to study the human Toll-like receptor 8 (hTLR8). This important pattern recognition receptor (PRR) recognizes - together with TLR7 - single-stranded (ss)RNA structures, a ...
HEK 293 cells are popular for their ease of growth and transfection (HEK293 Transfection Kit), making them a common cell culture in cancer
The study used HEK293 cells to develop a high cell-density perfusion method for recombinant human Erythropoietin protein production. Zika virus-like particles (VLPs): Stable cell lines and continuous perfusion processes as a new potential vaccine manufacturing platform ...
Pulix, M., et al., Molecular characterization of HEK293 cells as emerging versatile cell factories. Current Opinion in Biotechnology, 2021.71: p. 18-24. Alvim, R.G., I. Itabaiana Jr, and L.R. Castilho, Zika virus-like particles (VLPs): Stable cell lines and continuous perfusion process...
Yu P,Zhang L,Li SF,Li YP,Cheng JQ,Lu YR,Bu H.Long-term effects on HEK-293cell line after co-culture with porcine endogenous retrovirus. Transplantation Proceedings . 2005Long-term effects on HEK-293 cell line after co-culture with porcine endogenous retrovirus. Yu P,Zhang ...
HEK 293T Cell Culture Procedure General tips: 1.Cells are maintained at 37±1℃in a humidified atmosphere containing 5%±1% carbon dioxide. Split the cells once they grow to 70-90% confluency. 2.Typically, when splitting confluent HEK 293T cells in a 1:5 ratio, confluency is reached ...