Here, we describe a method for high throughput transient transfection of Human Embryonic Kidney 293 (HEK293) cells in 30 mL tubespins using polyethylenimine (PEI) as a transfection reagent. An automated liquid handler can be used to perform pipetting steps for transfecting batches of 96 tube...
Transfection efficiency was determined by qRT-PCR.Product Description:Transfection reagent is specially designed for HEK293 cellline, a human epithelial kidney cells. High efficiency delivering both DNA and RNA into HEK293 cells.Transfection Protocol and SDS:Download Altogen Biosystems HEK-293 Transfection...
Attachment 293 and 293/hTLR4-HA cultures were subjected to immunostaining according to the protocol outlined previously [10]. A mouse Anti-HA Tag primary antibody was used at a 1:1000 dilution to detect expression of the hTLR4-HA protein combined with an Alexa Fluor® 594 goat anti-mouse ...
TransfectionChinese hamster ovary (CHO) and human embryonic kidney 293 (HEK293) cells are the two most popular mammalian hosts for the production of recombinant proteins. In this chapter the suspension cultivation and transfection of these cells in small-scale disposable bioreactors is described. The...
Production methods based on transient gene expression are advantageous in terms of speed and versatility; yet, depending on the transfection protocol, transient transfection faces some bottlenecks such as a priori complex formation, limitations in terms of transfection and production media used and the ...
Attachment 293 and 293/hTLR4-HA cultures were subjected to immunostaining according to the protocol outlined previously [10]. A mouse Anti-HA Tag primary antibody was used at a 1:1000 dilution to detect expression of the hTLR4-HA protein combined with an Alexa Fluor® 594 goat anti-mouse ...
Transient transfections were performed in suspension in a 96-well plate using Metafectene PRO (Biontex) following the manufacturer’s protocol. Briefly, two solutions were prepared and incubated separately for 5 min. One solution contained plasmid DNAs encoding for BRET acceptor and donor diluted ...
Large-scale transfection of mammalian cells is now establishing itself as a "must-have" technology in the scientific community, as it allows the production of milligram to gram quantities of r-proteins within a few days after cDNA cloning into the appropriate expression vector. The HEK293 cell ...
Use the same growth medium for seeding, transfection and production Scalable protocol for use in flasks, spinner flasks and bioreactors Add complementary BalanCD HEK293 Feed and/or Anti-Clumping Supplement to maximize growth and titer Details
Use the same growth medium for seeding, transfection and production Scalable protocol for use in flasks, spinner flasks and bioreactors Add complementary BalanCD HEK293 Feed and/or Anti-Clumping Supplement to maximize growth and titer Fulfills regulatory requirements: ...