(GFP) as a reporter gene using calcium phosphate transfection method and imaging a large population of cells, we found that a 5-min DMSO exposure to HEK-293 cells, 4 hours after transfection of the protein of interest, leads to 1.6-fold increase in the expression yield without causing any ...
Profacgen's Technical Reference Guide for Recombinant Protein Expression in Mammalian Cells (HEK293/CHO) presents one of our current technical strategies, which can be used as a reference for protein production using the mammalian cell expression system.
set project goals with optimal results. Our recombinants have been successfully applied in R & D of therapeutics and diagnostics for years. Recombinants are preferably produced from the human cell line HEK293. HEK293 expressed proteins have superior product quality and activity due to post-translati...
Trending products:Recombinant Proteins|Bioactive Screening Libraries|Natural Products|Fluorescent Dye|PROTAC|Isotope-Labeled Compounds|Oligonucleotides 参考文献:[1]. Kang HW, et al. In vitro and In vivo imaging of antivasculogenesis induced by Noggin protein expression in human venous endothelial cells. F...
HEK293 cells are transfected with a plasmid for GFP expression with a high-efficient transfection. After 24 h of transfection, surviving cells are analyzed by flow cytometry. If the promoter strength and transfection efficiency are high enough, the GFP expression levels in the transfected cells ...
蛋白编号:Q13253 (Q28-C232) 基因ID:9241 同用名:Noggin; NOG 参考文献: [1]. Kang HW, et al. In vitro and In vivo imaging of antivasculogenesis induced by Noggin protein expression in human venous endothelial cells. FASEB J. 2009;23(12):4126-4134....
The results showed that the combination of CMV promoter and IFNα2 signal peptide resulted highly efficient for recombinant protein production in HEK293 cells. 展开 关键词: HEK293 cells Interferon-α2 Promoter Signal peptide Protein expression ...
(17). IL-2 is also a classically used cytokine for the expansion of NK cells, early differentiated T cells and effector memory Treg cells for adoptive cell transfer cancer immunotherapy (16, 18). GMP IL-2 is a commonly used supplement for the expansion of these cell types for cellula...
英文名称:HEK293T-EGFP (human embryonic kidney cells-green fluorescent protein marker (STR identification correct)) 英文同义词:HEK293T-EGFP (human embryonic kidney cells-green fluorescent protein marker (STR identification correct)) CAS号: 分子式: ...