1.Abaandou, Laura, David Quan and Joseph Shiloach. "Affecting Hek293 Cell Growth and Production Performance by Modifying the Expression of Specific Genes." Cells 10, no. 7 (2021): 1667. 2.Bloom, Fredric R, Paul Pric...
HEK293 is a cell line derived from human embryonic kidney cells grown in tissue culture. They are also known, more informally, as HEK cells. This particular line was initiated by the transformation and culturing of normal HEK cells with sheared adenovirus 5 DNA. The transformation resulted in t...
The HEK cell line has been extensively used as an expression tool for recombinant proteins since it was generated over 25 years ago. Although of epithelial origin, its biochemical machinery is capable of carrying out most of the post-translational folding and processing required to generate functiona...
However, 293 cells demonstrate no evident tissue-specific gene expression signature and express the markers of renal progenitor cells, neuronal cells and adrenal gland. This complicates efforts to reveal the authentic cell type/tissue of origin. On the other hand, the potential to propagate the ...
Centrosome amplification 参考文献 and the origin of chromosomal instability in breast cancer [J]. [1]Arquint C ,Sonnen KF ,Stierhof YD ,et al. Cell-cycle-regulated Journal of Mammary Gland Biology and Neoplasia ,2004 ,9 : expression of STIL controls centriole number in human cells[J]. ...
All cDNAs of human origin Cav3.x expression is under G418 selection (0.4 mg/ml maintenance, 1 mg/ml to prepare frozen aliquots) Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processe...
HEK293 clones expressing TLR2 gene of human or murine origin. 293/TLR2 clones are HEK293 cells stably transfected with a pUNO-TLR2 plasmid.
2 结果与讨论 2.1 ZEA 对 293 细胞的半数抑制率 ZEA 对 HepG2 细胞增殖力的抑制率呈计量相关 性,根据染毒浓度对应的细胞增殖抑制率,使用 Origin 8.0 进行 Polynomial Fit 分析,抑制率为 50%时对应的 浓度即为ZEA 对293 细胞的半数抑制浓度(IC50) 。ZEA 的浓度与抑制率曲线如图 1 所示,ZEAIC50=80 μM ...
The target gene (here GFP) is expressed under the control of CMV-pro. (C,D) Effect of DNA amount on GFP expression level in transfected cells. The GFP intensity (AU) of the cell population after 24 h of transfection was analyzed using flow cytometry. DNA amounts used in the ...
Mschen M,et al.(2002) The origin of CD95-gene mutations in B-cell lymphoma. Trends Immunol. 23(2): 75-80. Peter ME,et al.(2003) The CD95(APO-1/Fas) DISC and beyond. Cell Death Differ. 10(1): 26-35. Peter ME,et al.(2005) Does CD95 have tumor promoting activities Biochim...