Protocol for Recombinant Protein Expression using the Mammalian Expression System The following protocol can be a reference for both transient and stable gene expression using HEK293 cells. 1. Plasmid preparation a) Prepare pure plasmids for cell transfection could i. Maximize transfection efficiency ii...
Both our standard 3D cell culture protocol and the 3D static suspension culture protocols can be used for rapid cell expansion. The high-quality 3D spheroids enhance the cell performance for protein expression and downstream applications. HEK293 cells cultured in VitroGel HEK293 can easily be scale...
(2012) Expression of uncoupling proteins in a mammalian cell culture system (HEK293) and assessment of their protein function. Methods Mol. Biol. 810, 153-164Expression of uncoupling proteins in a mammalian cell culture system (HEK293)and assessment of their protein function. M. Jastroch. ...
Attachment 293 and 293/hTLR4-HA cultures were subjected to immunostaining according to the protocol outlined previously [10]. A mouse Anti-HA Tag primary antibody was used at a 1:1000 dilution to detect expression of the hTLR4-HA protein combined with an Alexa Fluor® 594 goat anti-mouse ...
Attachment 293 and 293/hTLR4-HA cultures were subjected to immunostaining according to the protocol outlined previously [10]. A mouse Anti-HA Tag primary antibody was used at a 1:1000 dilution to detect expression of the hTLR4-HA protein combined with an Alexa Fluor® 594 goat anti-mouse ...
Recombinant proteinSerum-free mediumSuspension cultureTransfectionTransient gene expression in human embryo kidney 293 (HEK293) cells is an established approach for the rapid production of large amounts of recombinant proteins (r-proteins). Milligram to gram......
Attachment 293 and 293/hTLR4-HA cultures were subjected to immunostaining according to the protocol outlined previously [10]. A mouse Anti-HA Tag primary antibody was used at a 1:1000 dilution to detect expression of the hTLR4-HA protein combined with an Alexa Fluor® 594 goat anti-mouse ...
When using expression vectors bearing the Epstein-Barr virus origin of replication, oriP (such as the pTT vector), a threefold improvement in r-protein yield is generally obtained over a similar non-oriP vector. This protocol describes a method for culturing HEK293-EBNA1 cells which will then...
Production methods based on transient gene expression are advantageous in terms of speed and versatility; yet, depending on the transfection protocol, transient transfection faces some bottlenecks such as a priori complex formation, limitations in terms of transfection and production media used and the ...
(2008) TMEM16A confers receptor-activated calcium-dependentchloride conductance. Nature 455: 1210-1215 Schroeder B.C. et al. (2008) Expression cloning of TMEM16A as a calcium-activatedchloride channel subunit. Cell 134: 1019-1029 Click here to download product pdf and Protocol...