Cell surface staining was performed on HEK293/Human c-MET&ErbB3 Stable Cell Line or negative control cell using APC-labeled anti-c-MET antibody and PE-labeled anti-ErbB3 antibody. Protocol Please contact us if you are interested in related cell pool service. Customer Reviews (0) + Writing ...
Note: The selection agent for the TLR4 stable line is blasticidin. Application(应用) The TLR4 stable cell line can be used for TLR4 flow cytometric calibration and detection control as well as TLR4-dependent functional assays. Product Handling Protocol(产品处理协议) Note: Please read the entire ...
We take advantage of this AAV unique feature to develop a rapid protocol to clone a stable recombinant protein expression human cell line. Findings We have constructed two plasmids. One plasmid, pSVAV2, contains the AAV rep gene for the synthesis of integrase; the second plasmid, pTRP5GFP...
(LZDK201703)Establishment of DOC-1R knockout HEK-293 stable cell line by CRISPR/Cas9 technologyWANG Shi-quan, GUO Ying-ying, SHEN Fei, GAO Jin-lan, XING Xue-sha, WANG Shu-sen, LUO Yang, *LIU Qi(The Research Center for Medical Genomics,the Key Laboratory of Medical Cell Biology,...
Transfection reagent is specially designed for HEK293 cellline, a human epithelial kidney cells. High efficiency delivering both DNA and RNA into HEK293 cells.Transfection Protocol and SDS:Download Altogen Biosystems HEK-293 Transfection Protocol: [PDF]...
Flow Chart of the Stable Expression Cell Line Development Protocol for Recombinant Protein Expression using the Mammalian Expression System The following protocol can be a reference for both transient and stable gene expression using HEK293 cells. 1. Plasmid preparation a) Prepare pure plasmids for cel...
Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 背景介绍 Human Embryonic Kidney 293 cells, also often referred to as HEK 293, 293 cells, or less precisely as HEK cells are a specific cell line originally derived from human embryonic kidney cells grown in ...
[1, 2]. This versatile human cell line benefts from a long history, extensive characterization, and successful protein expression in both transient and stable formats using plasmid and adenoviral vectors. In fact, in the case of the biopharmaceutical drug Xigris (activated Drotrecogin alfa), CHO ...
recombinant protein-expressing HEK293 cell line from serum-supplemented attachment culture to serum-free single cell suspension culture using the New Brunswick S41i CO 2 incubator shaker. This adapted HEK293 cell line behaves like a typical aggregationless suspension cell line. We expect that such ...
Sartorius offers a complete scalable bioreactor platform from cell and strain line development to commercial manufacturing. Explore Bioreactors & Fermenters FAQs - HEK 293 Media How do you grow viruses? What medium is used for cell culture in viral vector production? Why are HEK293 cells used fo...