Adaptation to suspension culture was carried out using multiple methods (Table 1). Each method is comprised of a commercially-available base medium to which standard cell culture supplements such as HI-FBS and L-glutamine were added according to the manufacturer’s adaptation protocol. In order to...
②:Supports 3D culture of human embryonic kidney 293 (HEK293) cells ③:Can be used for in vivo injection in animals 产品详情 VitroGel® HEK293is a gel product specifically developed for 3D culture of HEK293 cells. Whether using our standard 3D cell culture protocol or 3D static suspension ...
Cav3 Cell Culture Protocol Provider From the laboratory of Edward Perez-Reyes, PhD, University of Virginia. References Perez-Reyes, E., Van Deusen, A., Vitko, I. Molecular Pharmacology of Human Cav3.2 T-Type Ca2+ Channels: Block by Antihypertensives, Antiarrhythmics, and Their Analogs....
Adaptation to suspension culture was carried out using multiple methods (Table 1). Each method is comprised of a commercially-available base medium to which standard cell culture supplements such as HI-FBS and L-glutamine were added according to the manufacturer’s adaptation protocol. In order to...
Transfection reagent is specially designed for HEK293 cellline, a human epithelial kidney cells. High efficiency delivering both DNA and RNA into HEK293 cells.Transfection Protocol and SDS:Download Altogen Biosystems HEK-293 Transfection Protocol: [PDF]...
Host Cell: HEK293 Expressed gene: TLR7、NFκB-Luc Stability: 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) Freeze Medium: 90% FBS+10% DMSO Culture Medium: MEM+10%FBS+1% NEAA+1 mM NaP+100 μg/ml hygromycin+1 μg/ml puro...
Host Cell: HEK293 Expressed gene: TLR2 Stability: 32 passages (in-house test, that not means the cell line will be instable beyond the passages we tested.) Freeze Medium: 90% FBS+10% DMSO Culture Medium: MEM+10%FBS+1% NEAA+1 mM NaP+100 μg/ml hygromycin+1 μg/ml puromycin Mycop...
CellSensor® ISRE-bla HEK293T Cell-based Assay ProtocolComponentDMEM with GlutaMAXTMDialyzed FBSNEAAHEPES (pH 7.3)PenicillinStreptomycinBlasticidin antibioticRecoveryTM Cell Culture Freezing MediumCatalog no. KShipping: Dry IceStorage: Liquid Nitrogen...
What medium is used for cell culture in viral vector production? Why are HEK293 cells used for transfection? What media is most commonly used to culture HEK293 cells?Related Assets Application Note The Tested Protocol to Scale Up AAV Production With HEK293 Host Cells PDF | 389.7 KB Downloa...
When acclimating a cell line to suspension culture in preparation for scale-up to a stirred-tank bioreactor, it is important to confrm that protein expression was not impacted by the adaptation process. Whole cell lysates from untransfected 293 and 293/hTLR4-HA were analyzed for hTLR4-HA exp...