Cells directly thawed from liquid nitrogen or passaged from 2D culture vessels can be immediately mixed with the hydrogel solution for 3D static suspension cultures. Both our standard 3D cell culture protocol and the 3D static suspension culture protocols can be used for rapid cell expansion. The ...
Adaptation to suspension culture was carried out using multiple methods (Table 1). Each method is comprised of a commercially-available base medium to which standard cell culture supplements such as HI-FBS and L-glutamine were added according to the manufacturer’s adaptation protocol. In order to...
Adaptation to suspension culture was carried out using multiple methods (Table 1). Each method is comprised of a commercially-available base medium to which standard cell culture supplements such as HI-FBS and L-glutamine were added according to the manufacturer’s adaptation protocol. In order to...
Production of Recombinant Human Leukemia Inhibitory Factor (lif) in a Mammalian Cell Bioreactor: A First Approach Overall, this work will allow the establishment of a versatile platform for the production of a wide range of recombinant proteins to be used in stem cell culture in a cost-effective...
Transfection reagent is specially designed for HEK293 cellline, a human epithelial kidney cells. High efficiency delivering both DNA and RNA into HEK293 cells.Transfection Protocol and SDS:Download Altogen Biosystems HEK-293 Transfection Protocol: [PDF]...
询价 索取protocol 产品描述 产品数据库I. Background GLP-1R 与其他四种胰高血糖素受体(GCGR、GLP-2R、GIPR 和 GHRHR)一起属于 促胰液素 (B1 类) GPCR 家族,其内源性配体是肽激素。 B1 类 GPCRs 在 N 端具有一个较 大的、结构保守的胞外结构域(ECD),由 120-160 个残基组成,形成三层 α-β-β-...
When acclimating a cell line to suspension culture in preparation for scale-up to a stirred-tank bioreactor, it is important to confrm that protein expression was not impacted by the adaptation process. Whole cell lysates from untransfected 293 and 293/hTLR4-HA were analyzed for hTLR4-HA exp...
The mammalian HEK293 cell line provides a natural environment for expression of eukaryotic membrane proteins but optimization of transfection and cultivation time is often necessary to yield amounts of protein suitable for structural studies.Here we describe a detailed protocol for expression and ...
索取protocol 产品描述 产品数据库 I. Background GLP-1R 与其他四种胰高血糖素受体(GCGR、GLP-2R、GIPR 和 GHRHR)一起属于 促胰液素 (B1 类) GPCR 家族,其内源性配体是肽激素。 B1 类 GPCRs 在 N 端具有一个较 大的、结构保守的胞外结构域(ECD),由 120-160 个残基组成,形成三层 α-β-β-α 折...
Sartorius offers a complete scalable bioreactor platform from cell and strain line development to commercial manufacturing. Explore Bioreactors & Fermenters FAQs - HEK 293 Media Related Assets Application Note The Tested Protocol to Scale Up AAV Production With HEK293 Host Cells ...