Cells directly thawed from liquid nitrogen or passaged from 2D culture vessels can be immediately mixed with the hydrogel solution for 3D static suspension cultures. Both our standard 3D cell culture protocol and the 3D static suspension culture protocols can be used for rapid cell expansion. The ...
Whether using our standard 3D cell culture protocol or 3D static suspension culture, it enables rapid expansion of HEK293 cells. High-quality 3D cell spheroids facilitate protein expression and subsequent downstream applications. High-Density HEK293 Cell 3D Static Suspension Culture Preparing a high ...
Adaptation to suspension culture was carried out using multiple methods (Table 1). Each method is comprised of a commercially-available base medium to which standard cell culture supplements such as HI-FBS and L-glutamine were added according to the manufacturer’s adaptation protocol. In order to...
Adaptation to suspension culture was carried out using multiple methods (Table 1). Each method is comprised of a commercially-available base medium to which standard cell culture supplements such as HI-FBS and L-glutamine were added according to the manufacturer’s adaptation protocol. In order to...
This protocol describes a method for culturing HEK293-EBNA1 cells which will then be used to produce recombinant proteins. 展开 关键词: activated protein C tumor necrosis factor severe sepsis DOI: 10.1101/pdb.prot4976 被引量: 34 年份: 2008 ...
Transfection reagent is specially designed for HEK293 cellline, a human epithelial kidney cells. High efficiency delivering both DNA and RNA into HEK293 cells.Transfection Protocol and SDS:Download Altogen Biosystems HEK-293 Transfection Protocol: [PDF]...
When acclimating a cell line to suspension culture in preparation for scale-up to a stirred-tank bioreactor, it is important to confrm that protein expression was not impacted by the adaptation process. Whole cell lysates from untransfected 293 and 293/hTLR4-HA were analyzed for hTLR4-HA exp...
Infection protocolThe culture of HEK-293S cells in a stirred tank bioreactor for adenoviral vectors production for gene therapy is studied. Process monitoring using oxygen uptake rate (OUR) was performed. The OUR was determined on-line by the dynamic method, providing good information of the ...
Two protocols of HEK-293 cell line to RTCIT were tested (with and without virus adsorption) with different incubations times: 24, 48 and 72 h. The highest positive rate in HEK-293 (41 positive samples) resulted from the adsorption protocol with 72 h incubation period, in contrast to 43 ...
Culture Medium:DMEM+10%FBS+2μg/ml puromycin+100μg/ml hygromycin Mycoplasma Status:Negative Storage:Liquid nitrogen immediately upon delivery Application(s):Functional assay for CTR Transducer:Gs IV. Description of Host Cell Line Organism:Human ...