Add appropriate aliquots of the cell suspension to new culture vessels.Incubate cultures at 37°C....
a The cell viability of H9c2 cells was analyzed with CCK-8 assay. b Flow cytometry was applied to observe the apoptotic cells after staining with Annexin V-FITC/PI. c Western blot assay was performed to examine the expression of apoptosis-associated proteins. d The mRNA expression of MCP-1...
ATCC rat myocardial cell line h9c2Rat Myocardial Cell Line H9c2, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and morehttps://www.bioz.com/result/rat myocardial cell line h9c2/...
Cell viability assay The viability of the H9C2 rat cardiomyocytes was evaluated using a CCK-8 assay kit (MultiSciences Lianke), according to the manufacturer's protocol. H9C2 cells (1×104/well) seeded in 96-well plates were exposed to Hcy (0.5, 1, 2 or 3 mmol/l) for 24 h. For pro...
2.2. Cell culture and treatment Embryonic rat heart-derived cells (H9c2) were purchased from the Science Cell Excellence and Innovation Center of Chinese Academy of Sciences (Shanghai, China) and kept in a high glucose dulbeccos modified eagles medium (DMEM, 4.5 g/L glucose) supplemented with ...
Under both normal and low glucose cell culture conditions, neither DHA nor 19,20-EDP had any effect on citrate synthase and cytochrome c oxidase protein expressions (Fig. 3a–d). However, they significantly inhibited the catalytic activities when cultured with 25 mM glucose conditions (Fig. ...
B. Protocol used to investigate the effects of F2 on ROS/JNK/Egr-1 signaling in H9c2 cells after H/R. 1:10,000, respectively); IRDye incubations were performed in the dark. HRP-labeled secondary antibod- ies were detected using chemiluminescence, and the grayscale of the protein bands ...
Akt Activity Assay—Akt acitivity was assayed using an Akt assay kit (Cell Signaling Technology) according to the manufacturer's protocol. Briefly, Akt was immunoprecipitated from cell lysates using the anti-Akt antibody, and then the immunoprecipitates were incubated at 30 °C for 30 min in an...
Cell death analysis Fluorescein Annexin V-FITC/propidium iodide (PI) double labeling was performed using the Annexin V-FITC apoptosis detection kit (Beyotime Institute of Biotechnology, Shanghai, China), following the manufacturer’s protocol to detect the apoptosis of H9c2 cells subjected to the dif...
CELL PROLIFERATION TEST: The CCK8 method was used to detect the cells’ ability to proliferate. Cells from each group were seeded into 96-well plates at the same concentration and tested every 24 h. First, we removed the old culture medium and added 100 μL of fresh culture medium and 10...