Data and References 3D Cell Model Figure 1. 3D static suspension culture of HEK293 cells in VitroGel HEK293. Cells were mixed with VitroGel HEK293 at (2:1 gel/cells v/v ratio) and then mixed with cell culture medium at 1:3 ratio (cell-gel mixture: culture medium at 1:3 v/v). HE...
Origins of the HEK293 Cell Line HEK293 is a cell line derived from human embryonic kidney cells grown in tissue culture. They are also known, more informally, as HEK cells. This particular line was initiated by the transformation and culturing of normal HEK cells with sheared adenovirus 5 DNA...
Mammalian cells are often preferred over prokaryotic systems when expressing cDNAs of mammalian origin due to their superior capability to conduct elaborate post-translational modifications. Large-scale transfection of mammalian cells is now establishing itself as a "must-have" technology in the scientific...
保存条件:液氮保存纯度规格:1x10(6)viable cells/ml 产品类别:实验化学品 "HEK293-H Cell:人胚肾细胞系 细胞形态:上皮细胞样 细胞生长:贴壁 细胞背景资料:详见相关文献介绍 Porcine Kidney-15细胞系相关产品:PLA801C细胞、LS-1034细胞、8305C_1细胞
Culture medium supplementation with non-animal derived components including recombinant proteins and lipids of synthetic or non-animal-derived origin resulted in improved HEK 293 cell growth and VLP production. The maximum cell density attained using the optimized Freestyle culture medium was 5.4×106...
HEK293 clones expressing TLR2 gene of human or murine origin. 293/TLR2 clones are HEK293 cells stably transfected with a pUNO-TLR2 plasmid.
Even though there are many studies regarding clock resetting in culture using DEX, reference points or expression patterns of core clock genes and their protein products are scarce and sometimes contradict other works with similar methodology. We synchronise a cell line of human origin with DEX to ...
All cDNAs of human origin Cav3.x expression is under G418 selection (0.4 mg/ml maintenance, 1 mg/ml to prepare frozen aliquots) Voltage-sensitive calcium channels (VSCC) mediate the entry of calcium ions into excitable cells and are also involved in a variety of calcium-dependent processe...
Cells expressing a high level of GFP became spherical shaped and detached from the bottom of the culture dish (Fig. 1H,I), and these cells died after 72 h (data not shown). These cells were hardly observed in the empty vector transfection (Fig. 1G). Therefore, GFP overexpression (i....
—HEK-Blue™ hACE2-TMPRSS2 cells —HEK-Blue™ hACE2 cells These cells have been engineered to stably overexpress the host SARS-CoV-2 receptors, human (h)ACE2, and TMPRSS2 [1]. They were generated fromHEK‑Blue™ Null1‑v cells, which express an NF-κB inducible SEAP (secre...