3.Casademunt, Elisabeth, Kristina Martinelle, Mats Jernberg, Stefan Winge, Maya Tiemeyer, Lothar Biesert, Sigurd Knaub, Olaf Walter and Carola Schröder. "The First Recombinant Human Coagulation Factor Viii of Human Origin:...
Although of epithelial origin, its biochemical machinery is capable of carrying out most of the post-translational folding and processing required to generate functional, mature protein from a wide spectrum of both mammalian and non-mammalian nucleic acids. Though popular as a transient expression ...
The need for new safe and efficacious therapies has led to an increased focus on biologics produced in mammalian cells. The human cell line HEK293 has bio-synthetic potential for human-like production attributes and is currently used for manufacturing of
For transient transfection, the plasmid does not have any replication origin in cultured cells. The plasmid contains URA3 and 2µORI for recombination-based plasmid construction in the budding yeast Saccharomyces cerevisiae beside AmpR and pBR322ORI for selection and amplification in Escherichia coli....
All genes of mitochondrial origin across the electron transport chain (ETC) complexes exhibited at least a 2-fold increase in their expression (Figure S3A: genes with names starting with MT-). Apart from upregulation of mitochondrial genes, other genes that are expressed from the nuclear genome...
This band was not present in the other cell clones or in bulk HEK293 and HEK293T cells, and its origin is unclear. Download: Download high-res image (107KB) Download: Download full-size image Figure 2. Analysis of Knockout Clones by Western Blot Extracts from bulk HEK293 and HEK293T ...
HEK293 cells are the second most used cells in cell biology and the second most used mammalian cells in biopharmaceutical production [4,5]. They are used as platforms for the expression of recombinant proteins due to their high transfection efficiency, flexibility, and human origin [6,7,8,9]...
recombinant, expressed in HEK 293 cellsRecommended Products Slide 1 of 10 Sigma-Aldrich ZRB5535 Anti-SOX-9 Antibody, clone 2B10, ZooMAb® Rabbit Monoclonal recombinant, expressed in HEK 293 cells View Price and Availability Sigma-Aldrich SAB3500420 Anti-RIPK1 antibody produced in rabbit affinity...
Preferential transformation of human neuronal cells by human adenoviruses and the origin of HEK 293 cells. FASEB J. 16: 869-871, 2002. PubMed: 11967234 92579: Standard Test Method for Determining the Virus-Eliminating Effectiveness of Liquid Hygienic Handwash and Handrub Agents Using the Finger...
cells and was also required for their maintenance. Mechanistically, the 5-HT2A-mediated adhesion was mediated by downstream PKC and Rho signaling. Since 5-HT2Ais associated with many disorders such as dementia, depression and schizophrenia, its role in cell–matrix adhesion could have implications ...