Increasing demand for proteins with human-like PTMs, particularly viral proteins and vectors, have made human embryonic kidney 293 (HEK293) cells an increasingly popular host. The need to engineer more productive HEK293 platforms and the ongoing nature of the severe acute respiratory syndrome ...
The need for new safe and efficacious therapies has led to an increased focus on biologics produced in mammalian cells. The human cell line HEK293 has bio-synthetic potential for human-like production attributes and is currently used for manufacturing of several therapeutic proteins and viral vectors...
It is possible to foresee the S1/S2 site being catalysed by other proteases (including those of microbial origin), especially since enzymes that belong to the same family use common binding sites and have similar mechanistic features, which is defined by the nature of the nucleophilic amino acid...
For transient transfection, the plasmid does not have any replication origin in cultured cells. The plasmid contains URA3 and 2µORI for recombination-based plasmid construction in the budding yeast Saccharomyces cerevisiae beside AmpR and pBR322ORI for selection and amplification in Escherichia coli....
23,24,25] and provided the first descriptions of its three-dimensional structure by cryo-electron microscopy (cryo-EM) at 20–30 Å-resolution [26,27,28]. Subsequently, the production method in insect cells was improved by systematic optimization of the overexpression and purification steps ...
genetic modification of human pluripotent stem cells (hPSCs; including human embryonic and induced pluripotent stem cells) and the utilization of cardiomyocytes (CMs) derived thereof (hESC- or hiPSC-CMs) serves as an evolving technology to study physiological and pathophysiological functions of ion chan...
cells and was also required for their maintenance. Mechanistically, the 5-HT2A-mediated adhesion was mediated by downstream PKC and Rho signaling. Since 5-HT2Ais associated with many disorders such as dementia, depression and schizophrenia, its role in cell–matrix adhesion could have implications ...