Protocol for Recombinant Protein Expression using the Mammalian Expression System The following protocol can be a reference for both transient and stable gene expression using HEK293 cells. 1. Plasmid preparation a) Prepare pure plasmids for cell transfection could i. Maximize transfection efficiency ii...
In a previous study HIPK1, Homeodomain-interacting Protein Kinase 1, was found to be a target of the microRNA miR-22 that, when repressed, improved expression of both an intracellular and a secreted protein. In this report, HEK293 cells stably over-expressing miR-22 were compared with HEK...
For those who may be less familiar with it, Thermo Fisher Scientific defines its transient expression systems as those that can directly transfect, in bulk, suspension-adapted cells, typically either human embryonic kidney 293 cells (HEK293) or Chinese hamster ovary (CHO) cells. We...
产品编号 名称 包装 品牌 目录价 促销价 货期 数量 购物车 SB-HU5936 HEK293T-EGFP-PURO人胚肾细胞(荧光示踪细胞)+EGFP HEK293T-EGFP-PURO human embryonic kidney cells - green fluorescent protein labeling 1×10⁶cells/T25培养瓶 圣尔生物share-bio ¥3000.00 ¥3000.00 2个工作日 - + ...
A 50 µL suspension of the HEK-293T cells was seeded into wells of a sterile, white disposable 96-well flat-bottom microtitre plate (Greiner Bio-One, Germany) and left overnight in a 5% CO2 incubator at 37 °C. SARS-CoV-2 pseudovirion entry assay The protocol from BPS Bioscience ...
中文名称: Human Embryonic Kidney (HEK) 293 Cells Protein (Cell/Tissue Lysate) - LS-G134073 中文同义词: 英文名称: Human Embryonic Kidney (HEK) 293 Cells Protein (Cell/Tissue Lysate) - LS-G134073 英文同义词: Human Embryonic Kidney (HEK) 293 Cells Protein (Cell/Tissue Lysate) - LS-G13...
HEK293T-COGFP (human embryonic kidney cells-green fluorescent protein marker (STR identification correct))|HEK293T-COGFP(人胚肾细胞-绿色荧光蛋白标记(STR鉴定正确)) 基本信息 更多信息 中文名称: HEK293T-COGFP(人胚肾细胞-绿色荧光蛋白标记(STR鉴定正确)) 中文同义词: HEK293T-COGFP(人胚肾细胞-绿色...
Article: A reporter gene assay for high-throughput screening of G-protein-coupled receptors stably or transiently expressed in HEK293 EBNA cells gr
EMP Genetech provides non-GMP services in recombinant protein production and the development of stable high producer cell lines. Protein purification to customer given purity is also conducted, as well as cell banking. We work with industrial and academical clients. ...
Expression in HEK293 cells HEK293 cells (American Type Culture Collection) were grown in Dulbecco’s modified Eagle’s Medium (DMEM) (Gibco) supplemented with 10% fetal bovine serum (Gibco), 100 units/mL penicillin and 100 μg/mL streptomycin (Gibco) and incubated at 37 °C with 5% ...