Protocol for Recombinant Protein Expression using the Mammalian Expression System The following protocol can be a reference for both transient and stable gene expression using HEK293 cells. 1. Plasmid preparation a) Prepare pure plasmids for cell transfection could i. Maximize transfection efficiency ii...
In a previous study HIPK1, Homeodomain-interacting Protein Kinase 1, was found to be a target of the microRNA miR-22 that, when repressed, improved expression of both an intracellular and a secreted protein. In this report, HEK293 cells stably over-expressing miR-22 were compared with HEK...
The results showed that the combination of CMV promoter and IFNα2 signal peptide resulted highly efficient for recombinant protein production in HEK293 cells. 展开 关键词: HEK293 cells Interferon-α2 Promoter Signal peptide Protein expression ...
Transient expression in HEK 293 cells: an alternative to E. coli for the production of secreted and intracellular mammalian proteins. Transient transfection of kidney cells (293) enables the rapid and affordable lab-scale production of recombinant proteins. In this chapter protocols for t... JE Ne...
In this study, we developed an experimental method of systematically evaluating the expression limits of target proteins in the human embryonic kidney cell line HEK293 by measuring the target protein level in cells surviving after high-efficient, multicopy introduction of plasmid DNA by which the ...
EMP Genetech provides non-GMP services in recombinant protein production and the development of stable high producer cell lines. Protein purification to customer given purity is also conducted, as well as cell banking. We work with industrial and academical clients. ...
CD14 蛋白|CD14 protein|CD14(Mouse, His & Fc Tag)(产品说明) 蛋白名称:CD14 蛋白,CD14 protein 蛋白表达:CD14 protein is produced with HEK293 Cells. 蛋白纯度:CD14 protein purity > 97 % as determined by SDS
A 50 µL suspension of the HEK-293T cells was seeded into wells of a sterile, white disposable 96-well flat-bottom microtitre plate (Greiner Bio-One, Germany) and left overnight in a 5% CO2 incubator at 37 °C. SARS-CoV-2 pseudovirion entry assay The protocol from BPS Bioscience ...
We modified expression vectors based on pET, pFastBac and pTT backbones for parallel PCR-based cloning and screening in E.coli, insect cells and HEK293E cells, respectively. We introduced the toxic ccdB gene under control of a strong constitutive promoter for counterselection of insert less ...
Expression in HEK293 cells HEK293 cells (American Type Culture Collection) were grown in Dulbecco’s modified Eagle’s Medium (DMEM) (Gibco) supplemented with 10% fetal bovine serum (Gibco), 100 units/mL penicillin and 100 μg/mL streptomycin (Gibco) and incubated at 37 °C with 5% ...