Carrageenan (CGN) is widely used in the food manufacturing industry as an additive that stabilizes and thickens food products. Standard animal safety studies in which CGN was administered in diet showed no adverse effects. However, several in vitro studies have reported that intestinal inflammation is...
Patulin (PAT) is a kind of mycotoxins that commonly found in decayed fruits and their products. Our previous studies have shown that PAT induced cell apoptosis and the overproduction of reactive oxygen species (ROS) in human embryonic kidney (HEK293) cells. The present study aimed to further ...
It exists ubiquitously in agricultural products and daily food items such as cereals, beans, grapes, dried fruits, coffee, milk products, eggs, meat, fruits, and wine [4–7]. OTA has teratogenicity [8], nephrotoxicity [9–11], hepatotoxicity [12,13], cytotoxicity [14] and can cause a ...
Briefly, a series of concentrations of L655,240 was incu- bated with 250 nmol/L Rh-EVNLDAEFK-Quencher substrate and 0.02 unit/mL cathepsin D in reaction buffer (50 mmol/L sodium acetate, pH 4.5) for 1 h. Then, the fluorescence inten- sity of the enzymatic products was measured at ...
Mycotoxins are considered to be significant contaminants of food and animal feed. Zearalenone (ZEN) is a non-steroidal estrogenic mycotoxin produced by several species of Fusarium in cereals and agricultural products. ZEN has been shown to be cytotoxic, genotoxic, and mutagenic in different cell ...
Nevertheless, the US Food and Drug Administration approved PEG-IFNα2b in 2001 and PEG-IFNα2a in 2002 for the treatment of chronic hepatitis C virus infection. Another common problem associated with the use of refolded and pegylated IFNα (PEG-IFNα) is the formation of neutralizing ...
Patulin (PAT) is a mycotoxin produced by various fungal species that commonly contaminate apples and other fruit products. PAT is associated with glutathio
1,2 Increasing demand for gene-therapy products, with 359 in vivo therapies in clinical trials in the first half of 2020 and a predicted 10 to 20 approved cell and gene-therapy treatments by 2025, has created a need for manufacturing large quantities of rAAVs.3,4 However, current state-...
PCR products were Sanger sequenced and with these results, it was possible to determine the percentage of indels in the cellular pools by Synthego (https://ice.synthego.com/#/ (accessed on 10 October 2019)) online software tools to evaluate InDels in each region compared to control cells [...
2.5. Cell Viability Assay Functional food products which can be claimed as safe need to be confirmed on their viability effect of mammalian cells. The experiment was operated on human embryonic kidney cells, HEK293, to define the dose of extracts indicated as safe to the cells to be used ...