[7] T.A. M. Bharat, C. J. Russo, J. Lowe, L. A. Passmore, S. H. W. Scheres, Advancesin Single-Particle Electron Cryomicroscopy Structure Determination applied toSub-tomogram Averaging. Structure 23, 1743-1753 (2015).[8] B.A. Himes, P. Zhang, emClarity: software for high-resolu...
[7] T.A. M. Bharat, C. J. Russo, J. Lowe, L. A. Passmore, S. H. W. Scheres, Advancesin Single-Particle Electron Cryomicroscopy Structure Determination applied toSub-tomogram Averaging. Structure 23, 1743-1753 (2015). [8] B.A. Himes, P. Zhang, emClarity: software for high-reso...
目前的Cryo-EM主要分为了三个不同但密切相关的方向:电子晶体学(electron crystallography),单粒子冷冻电镜(single-particle cryo-EM)和电子低温断层扫描(electron cryotomography,Cryo-ET)。在过去几年中,单粒子Cryo-EM引发了结构生物学的革命,并已成为一个新的主导学科。 单颗粒冷冻电镜简史▲▲▲ 实际上,要想直接...
Cryo-EM structure determination of B2R-Gqcomplexes To obtain the active structures of B2R in complex with Gqheterotrimer, two B2R full agonists bradykinin and kallidin were used for the complex formation. The human B2R protein was engineered by replacing the N-terminal 28 amino acids with a mal...
所以,我们需要首先将细胞或者其他生物样本在极低的温度下瞬间冷冻,锁住它们在那一刻的状态,然后才能在冷冻电镜下进行观察。这也是冷冻电子显微镜成像(cryo-electron microscopy,简称cryo-EM)中“冷冻”(cryo-)两个字的由来。 除了需要冷冻以外,生物样品在冷冻电镜领域的应用还受到另外一个重要限制,那就是样品并不能经...
[7] T.A. M. Bharat, C. J. Russo, J. Lowe, L. A. Passmore, S. H. W. Scheres, Advancesin Single-Particle Electron Cryomicroscopy Structure Determination applied toSub-tomogram Averaging. Structure 23, 1743-1753 (2015). [8] B.A. Himes, P. Zhang, emClarity: software for high-reso...
1c). Subsequently, we used the DNA–RNA scaffold, mimicking the elongation transcription bubble, for the cryo-EM structure determination of the Pol I elongating complex (Pol I EC). The human Pol I EC was resolved at 2.7 Å resolution (Extended Data Fig. 1). To resolve the more ...
笔记:cryosPArc: algorithms for rapid unsupervised cryo-em structure determination,程序员大本营,技术文章内容聚合第一站。
[7] T.A. M. Bharat, C. J. Russo, J. Lowe, L. A. Passmore, S. H. W. Scheres, Advancesin Single-Particle Electron Cryomicroscopy Structure Determination applied toSub-tomogram Averaging. Structure 23, 1743-1753 (2015). [8] B.A. Himes, P. Zhang, emClarity: software for high-reso...
Cryo-EM structure determination captures new chemical modification of protein chemicalalonemodificationWith the discovery of the"central dogma"of life science,the molecular mechanism of genetics and evolution of biological species has basically been established.However,the completion of the Human Genome ...