Protein structure modeling is a fundamental step for the structural interpretation of 3D electron microscopy (EM) density map. Recently, because of the significant progress of the cryo-EM technique, protein structure modeling tools are needed for EM maps determined around 4 resolution. At this rear...
Single-particle electron cryo-microscopy (cryo-EM) is a powerful method for solving the three-dimensional structures of biological macromolecules. The technological development of transmission electron microscopes, detectors and automated procedures in c
Creative Biostructure provides cryo-EM services for the structure determination of Protein-Ligands complex.
Cryogenic electron microscopy (cryo-EM) is a mainstream technology for studying the structure of biological specimens such as cells, viruses, and proteins. It can provide three-dimensional (3D) maps of biomolecules with near-atomic resolution. Cryo-EM overcomes the deficiency of transmission electron...
Progress in cryo-electron microscopy has provided the potential for large-size protein structure determination. However, the success rate for solving multi-domain proteins remains low because of the difficulty in modelling inter-domain orientations. Here we developed domain enhanced modeling using cryo-...
leading expertise and innovation in high-resolution structural enablement by cryo-EM with a worldwide leader in protein production and characterization. Together, we aim to successfully target the most complex and challenging projects in drug discovery for our shared clients in a time-effective manner...
and Cube Biotech, a pioneer in membrane protein production and purification technologies, have announced a partnership agreement to develop a streamlined, cryo-EM enabled gene-to-structure workflow, to ultimately accelerate medicinal chemistry analysis and revolutionize the field of membrane protein research...
Zhang, Y. et al. Cryo-EM structure of the activated GLP-1 receptor in complex with a G protein.Nature546, 248–253 (2017). ArticleADSCASPubMedPubMed CentralGoogle Scholar Wootten, D. et al. The extracellular surface of the GLP-1 receptor is a molecular trigger for biased agonism.Cell16...
目前的Cryo-EM主要分为了三个不同但密切相关的方向:电子晶体学(electron crystallography),单粒子冷冻电镜(single-particle cryo-EM)和电子低温断层扫描(electron cryotomography,Cryo-ET)。在过去几年中,单粒子Cryo-EM引发了结构生物学的革命,并已成为一个新的主导学科。
GPCR structure determination G protein-coupled receptors(GPCRs) compose a large class of membrane proteins that are promising therapeutic targets for a variety of diseases, but they are notoriously difficult to crystallize, hampering crystallographic characterization. Cryo-EM has made significant headway ...