Here, we determined cryo-electron microscopy structure of human DNA-PK holoenzyme at 6.6 Å resolution. In the complex structure, DNA-PKcs, KU70, KU80 and DNA duplex form a 650-kDa heterotetramer with 1:1:1:1 stoichiometry. The N-terminal α-solenoid (∼2 800 residues) of DNA-PKcs...
RNA polymerase I (Pol I) specifically synthesizes ribosomal RNA. Pol I upregulation is linked to cancer, while mutations in the Pol I machinery lead to developmental disorders. Here we report the cryo-EM structure of elongating human Pol I at 2.7 Å re
Sui, Cryo-EM structure of SNAP-SNARE assembly in 20S particle, Cell Res. 25 (2015) 551-560, http://dx.doi.org/10. 1038/cr.2015.47.Zhou Q, Huang X, Sun S, Li X, Wang H-W, Sui S-F. 2015. Cryo-EM structure of SNAP-SNARE assembly in 20S particle. Cell Research 25:551-560....
22. Scheres, S. H. A Bayesian view on cryo-EM structure determination.Journal of molecular biology415, 406-418 (2012). 23. Scheres, S. H. RELION: implementation of a Bayesian approach to cryo-EM structure determination. Journal of structural biology180, 519-530 (2012). 24. Clapham, D....
Figure 2. Cryo-EM Structure of T. maritima Mg2+ Channel CorA in the Presence of Mg2+ at 3.8-Å Resolution (A) Side view of density map and fitted coordinates in ribbon representation of the entire pentamer with each protomer shown in a different color (A, orange; B, magenta; C, bl...
在进行抗体药物研发时,当冷冻电镜的数据分辨率比较低,可以通过测序信息来辅助进行序列鉴定。 简介 今天带来一篇发表在2022年1月份的science advance文献。 感染或疫苗接种之后的免疫应答分析是非常费时费力。ELISA或病毒中和实验可以带来非常丰富的信息,但是需要应对庞大的生物试剂...
应用冷冻固定术,在低温下使用透射电子显微镜观察样品的显微技术,就叫做冷冻电镜(CryoEM)。冷冻电镜是...
英语翻译 The cryo-EM structure of the T4S system core complex revealed a double-walled structure in the cap of the O layer (Supplementary Fig.10a)12.The crystal structure of the cap is not double-walled.This is because the lipid moiety of TraN/VirB7 was not built (the single-wavelength...
Figure 1. ASFV overall structure. A, cryo-images of ASFV virions with some Percoll particles derived from the purification process (see “Experimental procedures”); white arrowheads mark the outer envelope of the virion (in some cases broken); magenta arrowheads mark the inner membrane. Scale ...
resolution cryo-electron microscopy structure of the TV virion. The TV virion is about 400 ? in diameter and consists of a T = 3 icosahedral protein capsid enclosing the RNA genome. 180 copies of the major capsid protein VP1 (~57 KDa) are organized into two types of dimers A/B and C/...