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RNA polymerase I (Pol I) specifically synthesizes ribosomal RNA. Pol I upregulation is linked to cancer, while mutations in the Pol I machinery lead to developmental disorders. Here we report the cryo-EM structure of elongating human Pol I at 2.7 Å re
Here, we report the cryo-EM structure of the human translocase TIM22 complex at an overall resolution of 3.7 angstrom. The core subunit, Tim22, contains four transmembrane helices, forming a partial pore that is open to the lipid bilayer. Tim29 is a single transmembrane protein that provides...
2020年9月21日,北京生命科学研究所郑三多教授团队提交在 bioRxiv预印本上的“Cryo-EM structure of S-Trimer, a subunit vaccinecandidatefor COVID-19”,分别解析了新冠病毒S蛋白三聚体的野生型(WT)和突变型(MT)冷冻电镜结构。该项工作的冷冻电镜数据收集全部在水木未来电镜平台完成,WT和MT S-Trimers的Cryo-EM...
Figure 1. Structure determination and characterization of CVB1 particles before and after binding of CAR (A–D) Radially colored surface views of the cryoEM density maps of the CVB1 mature virion (A), the empty particle (B), the CVB1-M:CAR_37 complex (C) and the CAR-triggered A-parti...
Fig. 1: Cryo-EM structure of a full-length human ClC-2 homodimer. a Front view and top view of ClC-2 cryo-EM density map in homodimer form. Two protomers are shown in brown and purple, respectively. b Front view and top view of the ClC-2 atomic model. Color scheme is the same ...
μ-Opioid Receptor cryo-EM structure with nanobody antagonists The μ-opioid receptor (μOR) is a prototypical member of the G protein-coupled receptor (GPCR) family. Due to the recent opioid crisis, there is an interest in developing new modulators of μOR function. Andreas Borland’s lab...
Identification of mating type genes in the bipolar basidiomycetous yeast Rhodosporidium toruloides: first insight into the MAT locus structure of the Spori... Rhodosporidium toruloides is a heterothallic, bipolar, red yeast that belongs to the Sporidiobolales, an order within a major lineage of...
Cryo-EM Structure of Csm3/Tof1, Mrc1, and Ctf4 in Complex with CMG at a Replication Fork To assemble complexes for cryo-EM, we incubated S. cerevisiae CMG with fork DNA, Ctf4, Csm3/Tof1, Mrc1, and the non-hydrolyzable ATP analog adenylyl-imidodiphosphate (AMP-PNP) (Figure S1A). ...
Structure of the M2 muscarinic receptor–β-arrestin complex in a lipid nanodisc Here we present a cryo-electron microscopy (cryo-EM) structure of β-arrestin 1 (βarr1) in complex with muscarinic acetylcholine-2-receptor (M2R) reconstituted in lipid nanodiscs. The M2R–βarr1 structure shows...