15. Zhang, X., Jin, L., Fang, Q., Hui, W. H. & Zhou, Z. H. 3.3 Å cryo-EM structure of a nonenveloped virus reveals a priming mechanism for cell entry.Cell141, 472-482 (2010). 16. Mooney, P. Optimization of image collection for cellular electron microscopy.Methods in cell ...
Simian immunodeficiency viruses (SIVs) are lentiviruses that naturally infect non-human primates of African origin and seeded cross-species transmissions of HIV-1 and HIV-2. Here we report prefusion stabilization and cryo-EM structures of soluble envelope (Env) trimers from rhesus macaque SIV (SIV...
RNA polymerase I (Pol I) specifically synthesizes ribosomal RNA. Pol I upregulation is linked to cancer, while mutations in the Pol I machinery lead to developmental disorders. Here we report the cryo-EM structure of elongating human Pol I at 2.7 Å re
Accelerated cryo-EM structure determination with parallelisation using GPUs in RELION-2. eLife 5, e18722 (2016). Article PubMed PubMed Central Google Scholar Scheres, S. H. W. RELION: implementation of a Bayesian approach to cryo-EM structure determination. J. Struct. Biol. 180, 519–530...
无囊膜病毒大多数就像一个模子刻出来的，典型的结构是正二十面体组装（图1），其结构解析主要使用冷冻电镜成像技术cryo-EM及单颗粒重构方法SPA。而绝大多数囊膜病毒组装松散，柔性极大，高分辨率重构整颗囊膜病毒的唯一方法，就是采用冷冻电镜断层成像技术cryo-ET和子断层图像平均法STA。 为了解析囊膜病毒结构，Fuller...
Cryo-EM structure of NbE-µOR complex and close up of the NbE-µOR interaction interface. Cryo-EM of human fucosidase Close up of the FucA1 catalytic center without (A) and with (B) a bonded ligand. (C) shows several bacterial fucosidases overlaid onto the structure of FucA1. Fig...
“cryo-EM”) services to the pharmaceutical and biotechnology communities, andCube Biotech, a pioneer in membrane protein production and purification technologies, have announced a partnership agreement to develop a streamlined, cryo-EM enabled gene-to-structure workflow, to ultimately accelerate medicinal...
所以,我们需要首先将细胞或者其他生物样本在极低的温度下瞬间冷冻,锁住它们在那一刻的状态,然后才能在冷冻电镜下进行观察。这也是冷冻电子显微镜成像(cryo-electron microscopy,简称cryo-EM)中“冷冻”(cryo-)两个字的由来。 除了需要冷冻以外,生物样品在冷冻电镜领域的应...
Structure based rational drug design Cryo-EM can enable you to visualize molecules across a range of sizes and can help you obtain time-critical insights for your drug discovery projects. Learn more For Research Use Only. Not for use in diagnostic procedures. ...
33 High-resolution cryo-EM structure of the Trypanosoma brucei 80S: a unique eukaryotic ribosome Eukaryotic 80S ribosomes of known structure are far more complex than their 70S bacterial counterparts. Those from Saccharomyces cerevisiae, Tetrahymena thermophila, and Triticum aestivum, for example, bear ...