coli DNA gyrase bound to dsDNA and to the NBTI molecule gepotidacin using cryo-EM. The structure of the entire complex was solved at 6.6 Å resolution and two conformations of the DNA-binding/cleavage domain in closed and pre-opening states were solved at 4.0 and 4.6 Å ...
近日,浙江大学张岩及北京大学张岩共同通讯在Cell 在线发表题为“Structure-based design of non-hypertrophic apelin receptor modulator”的研究论文,该研究报告了与三种具有不同信号谱的激动剂结合的APLNR-Gi1复合物的冷冻电镜(cryo-EM)结构。 #心血管#心血管疾病#北京大学#浙江大学#冷冻电镜 ...
Cryo-EM structures of 11-2F / MTA-bound human PRMT5/MEP50 complex and itsform resolved at 3.1 and 3.2 respectively show that 11-2F in the catalytic pocket shifts the cofactor-binding pocket away by ~2.0, contributing to positive cooperativity. Computational analysis predicts subtype specificity ...
EPU single particle data collection tutorial and demonstration 87 -- 11:45 App Single Particle Cryo-Electron Microscopy: From Sample to Structure 54 -- 20:07 App Beatriz Costa Gomes (Turing) 256 -- 32:13 App Progress in cryoEM: Better, Faster, Cheaper, Smarter 52 -- 7:00 App vacuum...
2023年10月2日,美国普渡大学Daisuke Kihara研究组(第一作者为王虓)在Nature Methods上发表了文章CryoREAD: de novo structure modeling for nucleic acids in cryo-EM maps using deep learning,研究人员开发了从冷冻电镜图中全自动建模DNA/RNA原子结构的软件CryoREAD。它能够在2-5 Å分辨率的冷冻电镜图中进行...
Structure of the M2 muscarinic receptor–β-arrestin complex in a lipid nanodisc Here we present a cryo-electron microscopy (cryo-EM) structure of β-arrestin 1 (βarr1) in complex with muscarinic acetylcholine-2-receptor (M2R) reconstituted in lipid nanodiscs. The M2R–βarr1 structure shows...
we have pushed the resolution limit of single-particle cryo-EM to ∼3.3 Å, allowing ab initio model building of full-atom structures and functional interpretation of the resulting models (Figures 1C–1E). In so doing, we obtained a 3.3 Å resolution structure of the primed penetration ...
The chromatin structure of the central core region of Schizosaccharomyces pombe centromeric DNA is unusual. This distinctive chromatin structure is associa... L G,Marschall,Clarke - 《Journal of Cell Biology》 被引量: 222发表: 1995年 Cryo-EM structure of Schizosaccharomyces pombe Atg9 The chromatin...
point-cloud registration cryo-em 3d rmsd cryoem point-cloud-registration structure-comparison scale-transformations Updated Jun 9, 2022 Python I2PC / xmipp Star 37 Code Issues Pull requests Discussions Xmipp is a suite of image processing programs, primarily aimed at single-particle 3D electron...
在这种方法中,先验知识可以帮助减少数据不足时的不确定性,并提高结构解析的准确性。通过引入贝叶斯方法,EPFL-CryoEM为冷冻电镜领域提供了一种新的结构测定方法,为解决生物大分子结构研究中的挑战性问题提供了新的思路和工具。Implementation of a Bayesian approach to cryo-EM structure determination ...