This paper describes outcomes of the 2019 Cryo-EM Model Challenge. The goals were to (1) assess the quality of models that can be produced from cryogenic electron microscopy (cryo-EM) maps using current modeling software, (2) evaluate reproducibility of
We describe such a metric based on the fit of atomic models with independent test maps from single particle reconstructions not used in model refinement. The metric provides a means to determine the proper balance between the fit to the density and model energy and stereochemistry during refinement...
Cryo-EM model of the bullet-shaped vesicular stomatitis virus.Science2010,327,... JC Brown,WW Newcomb,GW Wertz - 《Viruses》 被引量: 21发表: 2010年 Atomic model of vesicular stomatitis virus and mechanism of assembly Like other negative-strand RNA viruses (NSVs) such as influenza and ...
Martin Pacesa of EPFL shows the development of a 3.3 Å reconstruction in under an hour using the novel Model Angelo modeling tool.
Fig. 1: The 3DFlex model represents the flexible 3D structure of a protein as deformations of a singlecanonical3D density mapV. Under the model, a single-particle image is associated with low-dimensional latent coordinates that encode the conformation for the particle in the image. A neural flo...
9. Henderson, R. & Unwin, P. N. T. Three-dimensional model of purple membrane obtained by electron microscopy.Nature257, 28 (1975). 10. Henderson, R. et al. Model for the structure of bacteriorhodopsin based on high-resolution electron cryo-microscopy.Journal of molecular biology213, 899-...
This paper describes outcomes of the 2019 Cryo-EM Model Challenge. The goals were to (1) assess the quality of models that can be produced from cryogenic electron microscopy (cryo-EM) maps using current modeling software, (2) evaluate re... CL Lawson,A Kryshtafovych,PD Adams,... - 《...
参考文献 M. Sitzmann, I. E. Weidlich, I. V. Filippov, et al., J. Chem. Inf. Model. 2012, 52(3), 739. Lee, S., Seok, C., Park, H., J. Comput. Chem. 2023, 44( 14), 1360.
This program can build models of large proteins (~1MDa) in a reasonable amount of time (~1day) and thus has the potential to greatly decrease the manual workload required for model building of high-resolution cryoEM maps.doi:10.1038/s41598-017-02725-wNiyun Zhou...
By fitting the previously determined high-resolution crystal structures of the component subunits into the cryo-EM map, we are able to provide an essentially complete model for the particle. This exposes SEPT2 NC-interfaces at the termini of the hexamer and leaves internal cavities between the ...