Interpreting electron cryo-microscopy (cryo-EM) maps with atomic models requires high levels of expertise and labour-intensive manual intervention in three-dimensional computer graphics programs1,2. Here we present ModelAngelo, a machine-learning approach for automated atomic model building in cryo-EM ...
CryoSPARC is a globally trusted complete solution for cryo-EM data processing Ultra-fast end-to-end processing of raw cryo-EM data and reconstruction of electron density maps, ready for ingestion into model building software Optimized algorithms and GPU acceleration at all stages, from pre-processing...
Advances in microscopy instruments and image processing algorithms have led to an increasing number of cryo-electron microscopy (cryo-EM) maps. However, building accurate models into intermediate-resolution EM maps remains challenging and labor-intensive. Here, we propose an automatic model building meth...
This repository provides a list of the state-of-the-art methods on (i) atomic model building from cryo-EM density maps, (ii) cryo-EM map sharpening, and (iii) de novo protein structure prediction. These methods leverage a combination of machine learning, deep learning, and other optimization...
CVPR 2015 paper "Building Proteins in a Day" Source Code New tools for automated high-resolution cryo-EM structure determination in RELION-3 eLife 7:e42166, bioRxiv 421123 "Accelerated cryo-EM structure determination with parallelisation using GPUs in RELION-2"eLife 5:e18722, bioRxiv 059717 "...
small-molecule positioning and model building (Fig.1a, Extended Data Figs.5and6, Extended Data Table1andSupplementary Discussion). The identities of β and γ subunits were determined from a combination of glycosylation patterns and side chain densities. Receptors in the two-Fab class had a tri-...
The crystallographic maps that are routinely used during the structure-solution workflow are almost always model-biased because model information is used for their calculation. As these maps are also used to validate the atomic models that result from model building and refinement, this constitutes an...
Cryo-EM model of the bullet-shaped vesicular stomatitis virus.Science2010,327,... JC Brown,WW Newcomb,GW Wertz - 《Viruses》 被引量: 21发表: 2010年 Viruses, Vol. 2, Pages 995-1001: Helical Virus Structure: The Case of the Rhabdovirus Bullet Commentary on Ge, P.; Tsao, J.; Schein,...
@article{li2023cryostar, author={Li, Yilai and Zhou, Yi and Yuan, Jing and Ye, Fei and Gu, Quanquan}, title={CryoSTAR: leveraging structural priors and constraints for cryo-EM heterogeneous reconstruction}, journal={Nature Methods}, year={2024}, month={Oct}, day={29}, issn={1548-7105...
This paper describes outcomes of the 2019 Cryo-EM Model Challenge. The goals were to (1) assess the quality of models that can be produced from cryogenic electron microscopy (cryo-EM) maps using current modeling software, (2) evaluate reproducibility of modeling results from different software de...