Advances in microscopy instruments and image processing algorithms have led to an increasing number of cryo-electron microscopy (cryo-EM) maps. However, building accurate models into intermediate-resolution EM maps remains challenging and labor-intensive. Here, we propose an automatic model building meth...
Interpreting electron cryo-microscopy (cryo-EM) maps with atomic models requires high levels of expertise and labour-intensive manual intervention in three-dimensional computer graphics programs1,2. Here we present ModelAngelo, a machine-learning approach for automated atomic model building in cryo-EM ...
The resolution of electron-potential maps in single-particle cryo-electron microscopy (cryoEM) is approaching atomic or near- atomic resolution. However, no program currently exists for de novo cryoEM model building at resolutions exceeding beyond 3.5 Å. Here, we present a program, EMBuilder...
2.1. Direct Atomic Model-Building Methods De novo atomic protein structure modeling for cryoEM density maps using 3D transformer and HMM Nabin Giri, Jianlin Cheng Nature Communications, June 2024. [paper][code][Cryo2Struct] Automated model building and protein identification in cryo-EM maps ...
CVPR 2015 paper "Building Proteins in a Day"Source Code New tools for automated high-resolution cryo-EM structure determination in RELION-3eLife 7:e42166, bioRxiv 421123 "Accelerated cryo-EM structure determination with parallelisation using GPUs in RELION-2"eLife 5:e18722, bioRxiv 059717 ...
CryoSPARC is a globally trusted complete solution for cryo-EM data processing Ultra-fast end-to-end processing of raw cryo-EM data and reconstruction of electron density maps, ready for ingestion into model building software Optimized algorithms and GPU acceleration at all stages, from pre-processing...
Cryo-electron microscopy (cryo-EM) has come a long way since the first viral structures were published by the Dubochet group in the early 1980s. Year over year, there is an almost exponential increase in the number of cryo-EM structures submitted to the Protein Data Bank (PDB), many at ...
The resolution of electron-potential maps in single-particle cryo-electron microscopy (cryoEM) is approaching atomic or near- atomic resolution. However, no program currently exists forde novocryoEM model building at resolutions exceedin... N Zhou,H Wang,J Wang - 《Scientific Reports》 被引量:...
By fitting the previously determined high-resolution crystal structures of the component subunits into the cryo-EM map, we are able to provide an essentially complete model for the particle. This exposes SEPT2 NC-interfaces at the termini of the hexamer and leaves internal cavities between the ...
Instead, a combination of modeling and fitting can be an effective way to analyze the EM structure at an atomic level, thus allowing localization of subunits or evaluation of conformational changes. Here we describe the steps involved in this process: building a homology model, fitting this model...