Likelihood‐based docking of models into cryo‐EM mapsdoi:10.1107/S2059798323001602Millán, ClaudiaMcCoy, Airlie J.Terwilliger, Thomas C.Read, Randy J.Acta Crystallographica: Section D, Structural Biology
e The cryo-EM map (left panels) and atomic model (right panels) of one layer of the GerQ filament. The dashed line in the bottom right panel indicates the direction of the split view displayed in (g). f The cryo-EM map and atomic model of one subunit of the GerQ filament. g ...
The homology model then was fitted to the cryo-EM map using the Fit In Map subroutine in UCSF Chimera (27). The vast majority of Cα-chains in the myosin-S1 motor domain, ranging from residues 216 to 624, matched densities visible in the reconstruction. Myosin loop 1 residues 199–216 ...
After fitting this homology model to a 17 resolution EM map, computational software is used to dock a metal-binding domain (MBD) that is unique to the copper pump. Although this software identifies a number of plausible interfaces for docking, the constraints of the EM map steer us to ...
First, MS-rings were identified from the 3D model. This consensus map was used to build a mask around the MS-ring. The MS-ring was then subtracted at the level of the micrograph (Extended Data Fig. 1a). The MS-ring-subtracted particles were then used to determine high-resolution ...
kuixu / awesome-cryoem Star 84 Code Issues Pull requests A collaborative list of awesome CryoEM (Cryo Electron Microscopy) resources. tomography electron-microscopy cryo-em 3d-reconstruction density-map model-building cryoem protein-structures 3d-denoise Updated on Jan 25, 2021 ...
b, c The cryo-EM density map b and atomic model c of SFTSV Gn/Gc heterodimer colored by domains, with the same code as in a. The N-linked glycans are colored in black, and the fusion loops are highlighted in green. The blue and black dashed boxes indicate the key interaction ...
using a whole-image beam-induced motion correction algorithm and many more particles, an even higher resolution was obtained for the 20S proteasome (another test sample for cryo-EM), which allowed for the first time to build an atomic model in a cryo-EM map from a particle with relatively ...
a, Composite cryo-EM map of the GR:Hsp90:FKBP51 complex. Hsp90A, dark blue; Hsp90B, light blue; GR, yellow; FKBP51, purple. Color scheme is maintained throughout. b, Atomic model in cartoon representation with boxes corresponding to the interfaces shown in detail in c–e. c, Interfac...
1b, mainly including sample preparation, cryo-EM grids setup and imaging, data collection and pre-processing, and 3D map reconstruction as well as the subsequent model building and structure analysis. Despite the low resolution of early electron microscopy, cryo-EM has been used in combination ...