备注：Emap2sec实现了一种基于CNN的算法，具有在整个3D图谱中执行局部结构检测的优势。EMBuild不仅在SPA电镜图谱（4-8 Å）上进行了评估，还在通过亚断层平均获得的图谱（4-9 Å）上进行了评估，并在将可靠的原子结构构建到中等分辨率3D图谱中显示出优异的性能。5. 冷冻电镜技术与人工智能的融合发展 冷冻电镜技...
They can be thresholded by specifying the expected false-discovery rate (FDR), and the displayed volume shows the parts of the map that have the corresponding level of significance. Here, the basic statistical concepts of confidence maps are reviewed and practical guidance is provided for their ...
Main Idea: Use 3D GAN to produce super-resolution 3D EM maps from an input low-resolution map Dataset: trained with dataset of pairs of an experimental EM map coupled with a corresponding high-resolution 3D simulated EM map that generated from the associated protein atomic structures. Generator:...
DEFMap only uses a cryo-EM map file for dynamics prediction and voxel visualization. Scale voxel length and map resolution cd data e2proc3d.py 015_emd_3984.map 015_emd_3984_5.0A_rescaled.mrc --clip=160,160,160 --scale=0.9 --process=filter.lowpass.gauss:cutoff_freq=0.2 # See the fo...
Post-process input map path/to/inputVol.mrc and save it at path/to/outputVol.mrc using high resolution deep learning model located in path/to/deep/learningModel deepemhancer -p highRes --deepLearningModelDir path/to/deep/learningModel -i path/to/inputVol.mrc -o path/to/outputVol.mrc ...
https://www.youtube.com/watch?v=Asp2Sg-AN-gWe show how to start building an atomic model into a cryoEM map using an atomic structure from the AlphaFold database. We fit the atomic model into the map a, 视频播放量 62、弹幕量 0、点赞数 5、投硬币枚数 1、收
On the right-hand side in Fig.1a, an example of local map alignment with EM maps of a complete V-ATPase structure (EMD-8724) and the Voregion (EMD-8409) is shown. The top panel shows vectors in the two maps (the number of vectors is reduced for illustration). Shown in the bottom...
map10. Thus, local sharpening methods have been proposed to overcome these limitations11,12. LocScale approach11compares radial averages of structure factor amplitudes inside moving windows between the experimental and the atomic density maps. After, the method modifies locally the map amplitudes of ...
DomainFit: Identification of protein domains in cryo-EM maps at intermediate resolution using AlphaFold2-predicted models DomainFit: Identification of protein domains in cryo-EM maps at intermediate resolution using AlphaFold2-predicted modelsdoi:10.1016/j.str.2024.04.017AlphaFold... J Gao,M Tong,C ...
An outer membrane secretin pore is required for passage of the pilus fiber out of the cell. Herein, the structure of the tetradecameric secretin, PilQ, from the Pseudomonas aeruginosa type IVa pilus system was determined to 4.3 and 4.4 resolution in the presence and absence of C7symmetric ...