但是由于结合抗体的多克隆性质和cryoEMPEM map的分辨率约为3到4埃,仅凭结构信息难以直接确定抗体序列。==作者假设如果能够获取适当的序列数据库==,在这个序列数据库中包含与采集血清(即pAb)的时间点相匹配的B细胞受体库(BCR),那么就可以利用cryoEMPEM中的含有的pAb的结构信息,从BCR库中选择出重链和轻链的候选者。
Main Idea: Use 3D GAN to produce super-resolution 3D EM maps from an input low-resolution map Dataset: trained with dataset of pairs of an experimental EM map coupled with a corresponding high-resolution 3D simulated EM map that generated from the associated protein atomic structures. Generator:...
https://www.youtube.com/watch?v=Asp2Sg-AN-gWe show how to start building an atomic model into a cryoEM map using an atomic structure from the AlphaFold database. We fit the atomic model into the map a, 视频播放量 62、弹幕量 0、点赞数 5、投硬币枚数 1、收
In addition, we also showed that some of the most recently proposed cryo-EM map post-processing algorithms can attenuate map anisotropy, thus offering alternative visualization opportunities for cases affected by moderate levels of preferential orientations.Sanchez-Garcia, Ruben...
density-modification procedure was applied to a set of 104 datasets and improved map-model correlation and increased the visibility of details in many of the maps. The procedure requires two unmasked half-maps and a sequence file or other source of information on the volume of the macromolecule ...
On the right-hand side in Fig.1a, an example of local map alignment with EM maps of a complete V-ATPase structure (EMD-8724) and the Voregion (EMD-8409) is shown. The top panel shows vectors in the two maps (the number of vectors is reduced for illustration). Shown in the bottom...
DiffFit: Visually-GuidedDifferentiableFitting of Molecule Structures to Cryo-EM Map If you use material from this repository, please cite the associated paper: Deng Luo, Zainab Alsuwaykit, Dawar Khan, Ondřej Strnad, Tobias Isenberg, and Ivan Viola. DiffFit: Visually-Guided Differentiable Fitting...
Post-process input map path/to/inputVol.mrc and save it at path/to/outputVol.mrc using high resolution deep learning model located in path/to/deep/learningModel deepemhancer -p highRes --deepLearningModelDir path/to/deep/learningModel -i path/to/inputVol.mrc -o path/to/outputVol.mrc ...
Symmetry-free cryo-EM structures of the chaperonin TRiC along its ATPase-driven conformational cycle Chaperonins are multisubunit entities that are composed of two stacked rings enclosing a central chamber for ATP-dependent protein folding. A series of cry... Y Cong,Gunnar F Schrder,AS Meyer,.....
An increasing number of cryo-electron microscopy (cryo-EM) density maps are being generated with suitable resolution to trace the protein backbone and guide sidechain placement. Generating and evaluating atomic models based on such maps would be greatly facilitated by independent validation metrics for ...