This approximation breaks down at very high resolution (<1 Å) where electron orbital structure becomes resolved and electron density peaks are distributed around nuclei [11]. In contrast, peaks in Coulomb potential maps are not necessarily centered on atomic nuclei, particularly when atoms carry ...
Enhancements in model building, refinement and computational simulation will be required to realize the full potential of EM-derived maps to reveal deeper insight into the electronic structure and functional properties of macromolecular complexes and their interactions with binding partners, ligands, ...
c, Surface representation of the C-ring density maps in the CCW pose superimposed on the final model. FliF subunits are shown in blue, FliG subunits are shown in red, FliM subunits are shown in yellow and FliN subunits are shown in shades of pink and purple. Source data Full size image...
Superimposed cryoEM maps are shown as blue mesh. Expanded top-down views of MAGEA4 pMHC molecule from the PN45545 TCR-CD3 complex (g) or the PN45428 TCR-CD3 complex (h). TCR residues that directly contact peptide residue D4 or HLA residue R65 are shown as orange (α) or purple (β...
Non-uniform refinement in cryoSPARC has been shown to significantly improve map quality when applied to maps with better than 8 Å resolution (Punjani et al., 2020). Masked refinement and local refinement are also common methods used to approach symmetry mismatches (D'Imprima et al., 2017, ...
structures presented in the Supplementary Table 1 of Pintilie et al.10, in which half maps are deposited have also been taken into account. Finally, the two recently deposited structures with higher resolution are included (EMD-11103 and EMD-11122). For this data set, the average absolute FSC...
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structures presented in the Supplementary Table 1 of Pintilie et al.10, in which half maps are deposited have also been taken into account. Finally, the two recently deposited structures with higher resolution are included (EMD-11103 and EMD-11122). For this data set, the average absolute FSC...
In recent years, advances in cryoEM have dramatically increased the resolution of reconstructions and, with it, the number of solved atomic models. It is widely accepted that the quality of cryoEM maps varies locally; therefore, the evaluation of the map
However, in those grids made at 200 ms and 6 s, the L31 subunit is absent within the EM maps when viewed at the same and lower threshold as the fast-plunge structures (Figure 6). During TED, chameleon, or Vitrobot grid preparation, shear forces acting on the sample may vary. Thus, ...