该原子模型和cryoEMPEM相比,在二级结构(Fig 2D Left)和侧链(Fig 2D Right)水平上呈现出很高的一致性。Per-residueQscore也很好的证实了Rh4O9.8抗体在Rh.4O9 map中展示出了很高的整体一致性(FW区和CDR区)。总而言之,基于B细胞分离出的Rh4O9.8 mAb和通过cryoEM鉴定出的pAb属于同一克隆家族成员。 Fig.2. 靶...
https://www.youtube.com/watch?v=Asp2Sg-AN-gWe show how to start building an atomic model into a cryoEM map using an atomic structure from the AlphaFold database. We fit the atomic model into the map a, 视频播放量 62、弹幕量 0、点赞数 5、投硬币枚数 1、收
早期的电镜技术根本无法研究生物结构,Henderson则用事实证明了其可行性,在70年代冷冻电镜技术尚未出现以前,便获得了七次跨膜螺旋结构。80年代冷冻电镜技术诞生以后,很多传统的结构生物学家依然不看好这项技术,甚至一度嘲笑其为“blobology”(英文讽刺...
CryoEM map of Pseudomonas aeruginosa PilQ enables structural characterization of TsaP The type IV pilus machinery is a multi-protein complex that polymerizes and depolymerizes a pilus fiber used for attachment, twitching motility, phage adsorption, natural competence, protein secretion, and surface-...
Elucidation of both the three-dimensional structure and the dynamics of a protein is essential to understand its function. Technical breakthroughs in single-particle analysis based on cryo-electron microscopy (cryo-EM) have enabled the three-dimensional
Figure 1: CryoEM map of ZIKV/ZIKV-117. From:A human antibody against Zika virus crosslinks the E protein to prevent infection The ZIKV-Fab surface is shown in radial colouring, from yellow (∼210 Å) to red (∼290 Å). The bound Fab (red ‘knobs’) extends from the ...
CryoEM map of Pseudomonas aeruginosa PilQ enables structural characterization of TsaP Author links open overlay panelMatthew McCallum 1 2 5 6, Stephanie Tammam 1 6, John L. Rubinstein 1 2 3 7, Lori L. Burrows 4 7 8, P. Lynne Howell 1 2 4 7Show more Add to Mendeley Share Cite...
Given that we cannot blindly accept the resolution of a structure reported by the FSC, assessing the quality of a cryo-EM map and the associated atomic model requires a comprehensive evaluation of numerous metrics. Although the topic of validation hasn't enjoyed the level of explosive development...
DiffFit: Visually-GuidedDifferentiableFitting of Molecule Structures to Cryo-EM Map If you use material from this repository, please cite the associated paper: Deng Luo, Zainab Alsuwaykit, Dawar Khan, Ondřej Strnad, Tobias Isenberg, and Ivan Viola. DiffFit: Visually-Guided Differentiable Fitting...