备注：Emap2sec实现了一种基于CNN的算法，具有在整个3D图谱中执行局部结构检测的优势。EMBuild不仅在SPA电镜图谱（4-8 Å）上进行了评估，还在通过亚断层平均获得的图谱（4-9 Å）上进行了评估，并在将可靠的原子结构构建到中等分辨率3D图谱中显示出优异的性能。5. 冷冻电镜技术与人工智能的融合发展 冷冻电镜技...
Main Idea: Use 3D GAN to produce super-resolution 3D EM maps from an input low-resolution map Dataset: trained with dataset of pairs of an experimental EM map coupled with a corresponding high-resolution 3D simulated EM map that generated from the associated protein atomic structures. Generator:...
但是由于结合抗体的多克隆性质和cryoEMPEM map的分辨率约为3到4埃,仅凭结构信息难以直接确定抗体序列。==作者假设如果能够获取适当的序列数据库==,在这个序列数据库中包含与采集血清(即pAb)的时间点相匹配的B细胞受体库(BCR),那么就可以利用cryoEMPEM中的含有的pAb的结构信息,从BCR库中选择出重链和轻链的候选者。
https://www.youtube.com/watch?v=Asp2Sg-AN-gWe show how to start building an atomic model into a cryoEM map using an atomic structure from the AlphaFold database. We fit the atomic model into the map a, 视频播放量 62、弹幕量 0、点赞数 5、投硬币枚数 1、收
2024年2月23日,来自华中科技大学黄胜友等研究人员合作在学术期刊《自然—生物技术》上发表了标题为“All-atom RNA structure determination from cryo-EM maps.”的研究成果,实现根据冷冻电镜图谱确定全原子RNA结构。 研究人员表示,从冷冻电镜图谱中确定蛋白质结构的方法很多,但对于RNA结构来说,这仍然是一项挑战。
map interpretationmap segmentationmodel‐buildingA procedure for building protein chains into maps produced by single﹑article electron cryo﹎icroscopy (cryo〦M) is described. The procedure is similar to the way an experienced structural biologist might analyze a map, focusing first on secondary ...
我们注意到该数据集包含的蛋白质数量非常少,但事实上DEFMap的输入是cryo-EM density map中的局部结构(尺寸为10Åx10Åx10Å,见图1),因此一个蛋白cryo-EM数据可以拆分成多个训练样本,再加上作者使用了随机旋转进行数据增强,整体数据量能够满足3D卷积神经网络的训练。
A density-modification procedure for improving maps from single-particle electron cryogenic microscopy (cryo-EM) is presented. The theoretical basis of the method is identical to that of maximum-likelihood density modification, previously used to improve
designed the neural networks and carried out model training and DEFMap analyses. S.M. and M.A. performed the MD calculations. S.M. and T.K. prepared the original cryo-EM map data. K.T. and Y.O. conceived the project. S.M. and S.I. wrote the manuscript. All of the authors dis...
DiffFit: Visually-GuidedDifferentiableFitting of Molecule Structures to Cryo-EM Map If you use material from this repository, please cite the associated paper: Deng Luo, Zainab Alsuwaykit, Dawar Khan, Ondřej Strnad, Tobias Isenberg, and Ivan Viola. DiffFit: Visually-Guided Differentiable Fitting...