model‐buildingA procedure for building protein chains into maps produced by single﹑article electron cryo﹎icroscopy (cryo〦M) is described. The procedure is similar to the way an experienced structural biologist might analyze a map, focusing first on secondary structure elements such as helices ...
T. gondiiis a model system for microtubule (MT) studies, as it contains at least five different types of tubulin-containing polymers, including the spindle (in replicating parasites), centrioles, the conoid, the intra-conoid MTs, and cortical MTs (also named subpellicular MTs)4(Fig.1a).T...
The comparison between the EMBuild model and the PDB structure is displayed in Fig. 6e. It can be seen from the figure that EMBuild succeeded in building a high-quality model on this subtomogram averaging map. The model built by EMBuild achieved the best quality among different methods. ...
Cryo-EM, cryo-ET, 3D reconstruction, and atomic model building A 3.5 μl aliquot of the LBP or HBP sample was applied to a holey grid and blotted for 4 s in a chamber at 80% humidity using an FEI Vitrobot Mark IV. Viruses were imaged with an FEI 300 kV Titan Krios cryoelectron mi...
Cryo-EM Structure Determination and Model Building To gain insight into mechanism of the dormant-to-primed state conversion, we obtained a 3.3 Å resolution structure of the metastable, primed aquareovirus ISVP by single-particle cryo-EM. The primed virus was prepared by removing the outermost ...
Experience in protein purification from E.coli, insect and mammalian cell culture Experience in the cryo-EM workflow (data collection, data processing, and model building) would be considered as plus Creative and self-motivated personality with an interest in fundamental and applied science ...
Tools for Model Building and Optimization into Near-Atomic Resolution Electron Cryo-Microscopy Density Maps Electron cryo-microscopy (cryoEM) has advanced dramatically to become a viable tool for high-resolution structural biology research. The ultimate outcome o... F Dimaio - 《Methods in Enzymology...
CryoSPARC is a globally trusted complete solution for cryo-EM data processing Ultra-fast end-to-end processing of raw cryo-EM data and reconstruction of electron density maps, ready for ingestion into model building software Optimized algorithms and GPU acceleration at all stages, from pre-processing...
CryoSPARC is a globally trusted complete solution for cryo-EM data processing Ultra-fast end-to-end processing of raw cryo-EM data and reconstruction of electron density maps, ready for ingestion into model building software Optimized algorithms and GPU acceleration at all stages, from pre-processing...
Overall, the evaluation against the density map indicated that the models are of high accuracy, and occasionally even better than the reference structure in some regions of the model. Local assessment of predicted side chains in a 1.52?? resolution map showed that side‐chains are sometimes ...