近日,来自字节跳动 ByteDance Research 团队的研究人员提出了 CryoSTAR 方法,成功将来自原子模型的结构先验应用到冷冻电镜实验数据的动态解析中,为解决这一问题带来了新的思路和方法。该成果相关论文 “CryoSTAR: Leveraging Structural Prior and Constraints for Cryo-EM Heterogeneous Reconstruction” 已刊发于 Nature 旗...
Henderson, R., and Unwin, P.N. (1975).Three-dimensional model of purple membrane obtained by electron microscopy.Nature 257, 28-32. Kuhlbrandt, W. (2014). Cryo-EM enters a newera. Elife 3, e03678.
不过,总的来说,将CCD相机引入cryo-EM的影响仍然很重要,因为它可以实现长时间连续自动化高通量的图像采集【17】。 将单粒子cryo-EM从低分辨率提升到原子结构测定的突破技术是直接电子检测相机(electron direct detection device,DDD)的引入【18】。2013年,程亦凡博士与David Agard (曾是Henderson 实验室的博士后) 把DD...
This paper describes outcomes of the 2019 Cryo-EM Model Challenge. The goals were to (1) assess the quality of models that can be produced from cryogenic electron microscopy (cryo-EM) maps using current modeling software, (2) evaluate reproducibility of
在Cryo-EM结构迅猛增长的背景下,分辨率的下降究竟会给基于结构的药物设计产生什么影响,有必要进行定量描述。下图为电镜解析出的一个蛋白质结构,黄色的区域为密度图,内部绿色的部分为拟合的蛋白结构。可以看到,在2.58Å的分辨率下,仅能看出肽链的大致走向,而侧链几乎很难在密度图中反映出来。
字节跳动 ByteDance Research 提出的新的结合结构先验的方法 (CryoSTAR) 显著提升了冷冻电镜(Cryo-EM)在动态构象解析中的应用潜力。通过利用原子结构模态先验作为约束,同时输出两种模态的解析结果,这一创新方法在技术上展示了其独特的优势,并为科学研究打开了新的大门。
This paper describes outcomes of the 2019 Cryo-EM Model Challenge. The goals were to (1) assess the quality of models that can be produced from cryogenic electron microscopy (cryo-EM) maps using current modeling software, (2) evaluate reproducibility of modeling results from different software ...
Ultra-fast end-to-end processing of raw cryo-EM data and reconstruction of electron density maps, ready for ingestion into model building software Optimized algorithms and GPU acceleration at all stages, from pre-processing through particle picking, 2D particle classification, 3D ab-initio structure ...
Cryo-electron microscopy (cryo-EM) has come a long way since the first viral structures were published by the Dubochet group in the early 1980s. Year over year, there is an almost exponential increase in the number of cryo-EM structures submitted to the Protein Data Bank (PDB), many at ...
Cryo-EM model of the bullet-shaped vesicular stomatitis virus (vol 327, pg 689, 2010) G Peng - 《Science》 被引量: 0发表: 2013年 Bluetongue virus coat prOtein VP2 contains sialic acid-binding domains, and VP5 resembles enveloped virus fusion proteins. a-helical bundle, similar to the fus...