Transcription of SARS-CoV-2 mRNA requires sequential reactions facilitated by the replication and transcription complex (RTC). Here, we present a structural snapshot of SARS-CoV-2 RTC as it transitions toward cap structure synthesis. We determine the atomic cryo-EM structure of an extended RTC ...
利用Cryo-FIB-SEM系统，研究人员还揭示了近天然状态下SARS-CoV-2在细胞内的组装和释放路径。研究结果表明，SARS-CoV-2感染的细胞病变效应在整个细胞水平上是深远的，例如在细胞质中有大量各种各样的囊泡用于RNA合成和病毒组装，其形成的膜通道有利于病毒释放或者从细胞质侵入细胞核。此外，还揭示了病毒RNA如何从双层...
Cryo-EM structure of an extended SARS-CoV-2 replication and transcription complex reveals an intermediate state in cap synthesis. Cell 184, 184–193.e10 (2021). Article CAS PubMed Google Scholar Hillen, H. S. Structure and function of SARS-CoV-2 polymerase. Curr. Opin. Virol. 48, 82–...
利用Cryo-FIB-SEM系统,研究人员还揭示了近天然状态下SARS-CoV-2在细胞内的组装和释放路径。研究结果表明,SARS-CoV-2感染的细胞病变效应在整个细胞水平上是深远的,例如在细胞质中有大量各种各样的囊泡用于RNA合成和病毒组装,其形成的膜通道有利于病毒释放或者从细胞质侵入细胞核。此外,还揭示了病毒RNA如何从双层膜泡...
Here we used cryo-electron microscopy (cryo-EM) to determine the structure of the post-fusion SARS-CoV S2 trimer. We present an analysis of the N-linked glycan shield masking the surface of the S2 machinery. Comparison with the structure of the pre-fusion SARS-CoV S glycoprotein reveals dra...
利用Cryo-FIB-SEM系统,研究人员还揭示了近天然状态下SARS-CoV-2在细胞内的组装和释放路径。研究结果表明,SARS-CoV-2感染的细胞病变效应在整个细胞水平上是深远的,例如在细胞质中有大量各种各样的囊泡用于RNA合成和病毒组装,其形成的膜通道有利于病毒释放或者从细胞质侵入细胞核。此外,还揭示了病毒RNA如何从双层膜泡...
cryoEMcryoETinactivated virusvaccinespikesubtomogram averagingprefusionpostfusionThe outbreak of SARS-CoV-2 in December 2019, led to the ongoing global pandemic of coronavirus disease 2019 (COVID19), which has claimed more than a half milli
Since the outbreak of the COVID-19 pandemic, cryo-electron microscopy (cryo-EM) and cryo-electron tomography (cryo-ET) have been demonstrated to be powerful and efficient tools in structural studies of distinct conformational states of the trimeric spike protein of SARS-CoV-2 and the VOCs as ...
However, the mechanism by which the Z protein regulates the activity of L polymerase is unclear. Here, we used cryo-electron microscopy to resolve the structures of both Lassa and Machupo virus L polymerases in complex with their cognate Z proteins, and viral RNA, to 3.1–3.9 Å ...
(Supplementary Fig. 6). We also solved the crystal structure of the NTD at a resolution of 2.2 Å. An atomic model of the uncleaved SARS-CoV S trimer was builtde novousing the 3.2 Å map, except the flexible RBD and part of the S1 NTD which were from fitted crystal ...