Transcription of SARS-CoV-2 mRNA requires sequential reactions facilitated by the replication and transcription complex (RTC). Here, we present a structural snapshot of SARS-CoV-2 RTC as it transitions toward cap structure synthesis. We determine the atomic cryo-EM structure of an extended RTC ...
The omicron variant of SARS-CoV-2 has been spreading rapidly across the globe. The virus-surface spike protein plays a critical role in the cell entry and immune evasion of SARS-CoV-2. Here we determined the 3.0 Å cryo-EM structure of the omicron spike protein ectodomain. In contrast...
Cryo-EM structure of an extended SARS-CoV-2 replication and transcription complex reveals an intermediate state in cap synthesis. Cell 184, 184–193.e10 (2021). Article CAS PubMed Google Scholar Hillen, H. S. Structure and function of SARS-CoV-2 polymerase. Curr. Opin. Virol. 48, 82–...
cryoEMcryoETinactivated virusvaccinespikesubtomogram averagingprefusionpostfusionThe outbreak of SARS-CoV-2 in December 2019, led to the ongoing global pandemic of coronavirus disease 2019 (COVID19), which has claimed more than a half milli
Article https://doi.org/10.1038/s41467-024-45356-2 Cryo-EM structure of the extracellular domain of murine Thrombopoietin Receptor in complex with Thrombopoietin Received: 1 September 2023 Accepted: 19 January 2024 Check for updates Kaiseal T. G. Sarson-Lawrence1,2,5, Joshua M. Hardy 1,2,...
Here we present high-resolution structures of the trimeric MERS-CoV and SARS-CoV S proteins in its pre-fusion conformation by single particle cryo-electron microscopy (cryo-EM). We captured two states of the RBD that is facilitated to receptor binding and further analysis of S proteins revealed...
The cryo-EM structure of Flycatcher1 revealed a heptameric architecture (Figure S7D) resembling that of the bacterial MscS and plant MSL1 channels, with both N and C termini intracellularly located [131]. Each Flycatcher1 subunit contains six TMs, of which TM1/2/3 are additional to MscS/MSL...
Researchers at Hokkaido University have been using a Krios G4 Cryo-TEM in BSL-3 conditions to study structures of pathogens like SARS-CoV-2 to support vaccine development. Cryo-EM in Biosafety Facilities for Virology Research Download brochure Learn more about cryo-EM ...
The BA.2 sub-lineage of the SARS-CoV-2 Omicron variant has gained in proportion relative to BA.1. As differences in spike (S) proteins may underlie differencesStalls, VictoriaLindenberger, JaredGobeil, SophieHenderson, RoryParks, RobBarr, Maggie...
To assess the spraying performance, we examined the percentage of intact proteins under different parameters using apo-ferritin as a testing model by negative staining EM and native MS analysis (Extended Data Figs. 1 and 2). To control the size of initial droplets generated from the ESI tip, ...