DNA replication in Eukaryotes is a highly dynamic process that involves several dozens of proteins. Some of these proteins form stable complexes that are amenable to high-resolution structure determination by cryo-EM, thanks to the recent advent of the direct electron detector and powerful image ...
G-protein-coupled receptors (GPCRs) activate heterotrimeric G proteins by stimulating guanine nucleotide exchange in the Gα subunit1. To visualize this mechanism, we developed a time-resolved cryo-EM approach that examines the progression of ensembles of pre-steady-state intermediates of a GPCR–G...
The synthesis of secretory or integral membrane proteins can be directly coupled to their translocation across or insertion into membranes. In co-translati... M Halic,R Beckmann - 《Current Opinion in Structural Biology》 被引量: 311发表: 2005年 The process of mRNA-tRNA translocation. protein ...
摘要: DNA replication in Eukaryotes is a highly dynamic process that involves several dozens of proteins. Some of these proteins form stable complexes that are amenable to high-resolutio... 查看全部>>关键词: Cryo-EM DNA replication eukaryotic DNA replication origin recognition complex replicative ...
Electron cryomicroscopy (cryo-EM) has evolved as a widely used approach to understand a range of structure-function questions, particularly of membrane proteins. Studies by both electron crystallography and single particle analysis have provided a wealth of information on membrane transport proteins. Cr...
Elucidation of both the three-dimensional structure and the dynamics of a protein is essential to understand its function. Technical breakthroughs in single-particle analysis based on cryo-electron microscopy (cryo-EM) have enabled the three-dimensional structures of numerous proteins to be solved at ...
This talk dives into the myriad ways in which cryo-EM is shaping the future of pharmaceutical research, showcasing its versatility and promise in addressing the pressing challenges of drug discovery and development. 0:00 High resolution electron cryo-microscopy: From membrane proteins to disease...
In the recent years, the protein databank has been fueled by the exponential growth of high-resolution electron cryo-microscopy (cryo-EM) structures. This trend will be further accelerated through the continuous software and method developments and the i
This talk dives into the myriad ways in which cryo-EM is shaping the future of pharmaceutical research, showcasing its versatility and promise in addressing the pressing challenges of drug discovery and development. 0:00 High resolution electron cryo-microscopy: From membrane ...
在一项新的研究中,来自英国医学研究理事会分子生物学实验室和德国海德堡大学的研究人员利用cryo-EM方法研究了S三聚体在病毒颗粒表面上的结构、构象和分布。相关研究结果于2020年8月17日在线发表在Nature期刊上,论文标题为“Structures and distributions of SARS-CoV-2 spike proteins on intact virions”。