将沉积和玻璃化之间的时间缩短到几十毫秒已被证明有助于保持蛋白质的完整性。 单颗粒分析技术(single-particle analysis)的三种沉积方法 为制备用于单颗粒分析的适当薄膜,人们采用了不同的方法(表 1)。这些方法可分为三类:印迹法、液滴法和划线法。 3.3.1 印迹法 Blotting 印迹法是目前最常
While a wide range of conditions have been used to prepare RNAs for cryo-EM analysis, correlations between the variables in these conditions and cryo-EM visualizations and reconstructions remain underexplored, which continue to hinder optimizations of RNA samples for high-resolution cryo-EM structure ...
Here we used cryo-electron microscopy (cryo-EM) to determine the structure of the post-fusion SARS-CoV S2 trimer. We present an analysis of the N-linked glycan shield masking the surface of the S2 machinery. Comparison with the structure of the pre-fusion SARS-CoV S glycoprotein reveals dra...
the necessary gating becomes a fascinating and critical functional aspect. To address this and further elucidate the structural basis for injectisome assembly and function, including that of the central needle component, we have conducted here a cryo-EM analysis of the assembled native needle complex...
Optimized algorithms and GPU acceleration at all stages, from pre-processing through particle picking, 2D particle classification, 3D ab-initio structure determination, high resolution refinement, and heterogeneity analysis Specialized and unique tools for therapeutically relevant targets, membrane proteins, co...
Single particle analysis cryo EM software for automated cryo electron microscopy data acquisition, enabling high-resolution 3D protein structure determination.
Experience how Smart EPU Software with Embedded CryoSPARC Live can analyze single particle analysis data in real-time on a Thermo Scientific cryo-TEM. 0:00 Ask the Experts In this webinar, we review the capabilities that have recently been added to Smart EPU Software, and how they can furthe...
Thermo Scientific Smart EPU Software is an AI-powered, cryo-electron microscopy (cryo-EM) software platform that automates data acquisition, optimizes grid screening, and seamlessly integrates with your hardware for accelerated single particle analysis data workflow. ...
turning plotfsc analysis script into a utility tool Mar 26, 2024 cryodrgn getting rid of Sphinx docs and dataset device usage Apr 11, 2025 testing better tests, documentation, and backwards compatibility Aug 17, 2024 tests adding separate random seed for data shuffler; adding compatibility w… ...
PAGE analysis shows that this process is Mg2+ dependent, since low [Mg2+] results in a change in intensity of each band in the ladder, especially that from tetramers and pentamers (top right). Densitometric quantification of band intensity (bottom right) shows that as [Mg2+] increases, ...