Cryo-electron microscopy (cryo-EM) captures snapshots of dynamic macromolecules, collectively illustrating the involved structural landscapes. This provides an exciting opportunity to explore the structural variations of macromolecules under study. Howev
Cryo-EMCryo-electron microscopySingle particle analysisThe quality of a 3D map produced by the single-particle analysis method is highly dependent on an accurate assignment of orientations to the many experimental images. However, the problem's complexity implies the presence of several local minima ...
“Thanks to cryo-EM and thanks to single particle analysis we can directly extract those [amyloid] filaments from the heart of a patient who died from amyloidosis and we could get high resolution information about the structure and the overall architecture of the filament.” ...
Electron cryo-microscopy (cryo-EM) single-particle analysis has yielded protein structures with increasing levels of detail in recent years1,2. However, it has proved difficult to obtain cryo-EM reconstructions with sufficient resolution to visualize individual atoms in proteins. Here we use a new ...
Dive deeper into the innovations behind Smart EPU Software and see how it can enable a more automated experience for cryo-EM single particle analysis data acquisition. Download datasheet Additional resources eBook: Getting started with cryo-electron microscopy Datasheet: Guidance for planning a cr...
单颗粒分析技术(single-particle analysis)的三种沉积方法 为制备用于单颗粒分析的适当薄膜,人们采用了不同的方法(表 1)。这些方法可分为三类:印迹法、液滴法和划线法。 3.3.1 印迹法 Blotting 印迹法是目前最常用的标准薄膜制备技术。这种技术历史悠久 ,直到最近才成为唯一的商业选择,如 Vitrobot(赛默飞世尔科技公...
“Thanks to cryo-EM and thanks to single particle analysis we can directly extract those [amyloid] filaments from the heart of a patient who died from amyloidosis and we could get high resolution information about the structure and the overall architecture of the filament.” ...
单颗粒分析技术(single-particle analysis)的三种沉积方法 为制备用于单颗粒分析的适当薄膜,人们采用了不同的方法(表 1)。这些方法可分为三类:印迹法、液滴法和划线法。 3.3.1 印迹法 Blotting 印迹法是目前最常用的标准薄膜制备技术。这种技术历史悠久 ,直到最近才成为唯一的商业选择,如 Vitrobot(赛默飞世尔科技公...
这样就可以获得几十万张这种蛋白质的照片,每个蛋白质的方向各不相同,相当于对同一个蛋白质拍摄了几十万张不同的投影照片。将这些照片的信号叠加在一起,就能提高信噪比,获得蛋白质的高清三维结构。这种方法被称为冷冻电镜单颗粒成像技术SPA(Single particle analysis)。
这样就可以获得几十万张这种蛋白质的照片,每个蛋白质的方向各不相同,相当于对同一个蛋白质拍摄了几十万张不同的投影照片。将这些照片的信号叠加在一起,就能提高信噪比,获得蛋白质的高清三维结构。这种方法被称为冷冻电镜单颗粒成像技术SPA(Single particle analysis)。