(i) cryo‐EM describes the usage of transmission electron microscopy for the visualization of a specimen in a thin layer of vitreous ice at very low temperature and (ii) single‐particle reconstruction describes two‐ (2D) and, preferably, three‐dimensional (3D) reconstruction of the specimen ...
Single-particle electron cryo- microscopy: towards atomic resolution. Q. Rev. Biophys. 33 (4), 307-369.Kastner, B. et al. GraFix: sample preparation for single-particle electron cryomicroscopy. Nat. Methods 5, 53-55 (2008).
In electron cryomicroscopy (cryo-EM), molecular images of vitrified biological samples are obtained by conventional transmission microscopy (CTEM) using large underfocuses and subsequently computationally combined into a high-resolution three-dimensional structure. Here, we apply scanning transmission electron...
Single-Particle Cryo-Electron Microscopy (Cryo-EM): Progress, Challenges, and Perspectives for Further Improvement Electron microscopy (EM) of biological macromolecules has experienced a significant increase in its capabilities with the introduction of a new generation ... D Agard,Y Cheng,RM Glaeser,...
Understanding the complexity of cancer with cryo-EM Download eBook Scientific webinars Hear from the scientific community using single particle cryo-EM to empower their research. View gallery Electron Microscopy Funding Support Center Access technical content, Thermo Fisher Scientific Financial Ser...
The fast development of single-particle cryogenic electron microscopy (cryo-EM) has made it more feasible to obtain the 3D structure of well-behaved macromolecules with a molecular weight higher than 300 kDa at ~3 Å resolution. However, it remains a challenge to obtain the high-resolution...
Understanding the complexity of cancer with cryo-EM Download eBook Scientific webinars Hear from the scientific community using single particle cryo-EM to empower their research. View gallery Electron Microscopy Funding Support Center Access technical content, Thermo Fisher Scientific Financial...
SingleParticle.com is the premium provider of turn-key cryo-EM computing solutions, including GPU workstations and compute/storage clusters.
Understanding the complexity of cancer with cryo-EM Download eBook Scientific webinars Hear from the scientific community using single particle cryo-EM to empower their research. View gallery Electron Microscopy Funding Support Center Access technical content, Thermo Fisher Scientific Financial Services, and...
Single-Particle Cryo-EM at Crystallographic Resolution Cell, Volume 161, Issue 3, 23 April 2015, Pages 450-457 Yifan ChengView PDFCryo-electron microscopy (cryo-EM) of single-particle specimens is used to determine the structure of proteins and macromolecular complexes without the need for crystals...