In principle, electron crystallography is a high-resolution technique and it has indeed been demonstrated in a number of cases that near-atomic resolution can be attained. Single-particle analysis is particularly suited for structural studies of large macromolecular complexes. The amount of material ...
Interestingly, filaments of different diameter have also been observed on the surface ofSynechocystis52. It will now be important to discover if this principle occurs in other bacterial species, and excitingly, will open avenues to the development of vaccines or therapeutics targeting a particular T4P...
Cryo-electron microscopy(cryo-EM) is based on the principle of imaging radiosensitive objects in a transmissionelectron microscopeunder cryogenic conditions. Electrons, accelerated toward the sample by electrical potential differences, interact with themacromoleculesembedded in the sample. As a result of ...
While cryo-electron microscopy encompasses a number of experimental methods (imaging intact tissue sections, imaging plunge frozen cells and virus etc), these methods are based on the principle of imaging radiation-sensitive specimens in a transmission electron microscope....
Cryo-electron microscopy is an essential tool for high-resolution structural studies of biological systems. This method relies on the use of phase contrast imaging at high defocus to improve information transfer at low spatial frequencies at the expense of higher spatial frequencies. Here we demonstrat...
all of these light microscopes have their limits when it comes to magnification. On this border electron microscopy steps in which allows to see even smaller structures which are restricted for light microscopes. The limits of both worlds, light and electron microscopy have been pushed in the past...
Negative stain electron microscopy showed that the mutant could assemble Aap pili but not threads, confirming our structural data (Supplementary Fig. 1f). Using the sequence of Saci_0406, we were able to build an atomic model for the thread unambiguously (Fig. 1b–e), excluding the N-...
Cryo-EM datasets were deposited in the Electron Microscopy Public Image Archive under accession codes EMPIAR-11745. The reconstructed cryo-EM maps were deposited in the Electron Microscopy Data Bank with the accession codes: Morphology-1a: EMD-18715; Morphology-1b: EMD-18716; Morphology-2: EMD-...
Cryo-electron microscopy FBDD: Fragment-based drug discovery GLP-1R: Glucagon-like peptide-1 receptor GPCR: G-protein-coupled receptor ML: Machine learning NMR: Nuclear magnetic resonance NPC: Nuclear pore complex PKM2: Pyruvate kinase isozymes M2 PROTAC: Proteolysis targeting chimera ...
cryo-electron microscopy nuclear pore complex cryo-electron tomography in situ structure The compartmentalization of eukaryotic cells enables organelles to operate separately to perform diverse functions. One of the largest compartments is the nucleus, which contains the genetic materials.1 A giant protein...