Cryo-Electron Microscopy (ANDS Tech Talk).pdfWilson, Lance
Intractable,Cryo-ElectronMicroscopy,StructuralBiology 1Introduction Structuralbiologystudieshowbiologicalsystemsarebuilt.Especially,deter- miningthree-dimensionalelectrondensitymapsofmacromolecularcomplexes, suchasproteinsorviruses,isoneofthemostimportanttasksinstructural biology[15]. Standardtechniquestoobtainthree-dimensiona...
Cryo-electron microscopy (cryo-EM) of non-crystalline single particles is a biophysical technique that can be used to determine the structure of biological macromolecules and assemblies. Historically, its potential for application in drug discovery has been heavily limited by two issues: the minimum ...
Sharpening high resolution information in single particle electron cryomicroscopy 下载文档 收藏 打印 转格式 35阅读文档大小:830.15K6页gysdvr72上传于2015-05-30格式:PDF high-resolution single particle analysis from electron cryo-microscopy images using sphire.[2017][j vis exp][10.379155448] ...
在现代电子显微镜中,通常使用场发射源,又称电子枪(electron guns)或者场发射枪(FEGs)。一般有两种类型:肖特基场发射枪(Schottky FEG)以及冷场发射枪(cold FEG)。场发射枪的原理在这里不再赘述,简单来说相比于热发射,使用了超级小的尖端(半径100nm左右)来增强电场强度,第一个阳极用于促进电子逸出、第二个阳极用于...
Here, we determined cryo-electron microscopy structure of human DNA-PK holoenzyme at 6.6 Å resolution. In the complex structure, DNA-PKcs, KU70, KU80 and DNA duplex form a 650-kDa heterotetramer with 1:1:1:1 stoichiometry. The N-terminal α-solenoid (∼2 800 residues) of DNA-PKcs...
In my contribution I will focus on the application of two biophysical methods, time-resolved X-ray scattering from solutions using synchrotron radiation (see Mandelkow et al., 1980; Bordas et al., 1983) and cryo-electron microscopy of frozen-hydrated molecules (Mandelkow & Mandelkow, 1985)....
内容提示: REVIEWSingle-particle cryo-EM—How did itget here and where will it goYifan ChengCryo–electron microscopy, or simply cryo-EM, refers mainly to three very different yetclosely related techniques: electron crystallography, single-particle cryo-EM, andelectron cryotomography. In the past ...
PDF (15759 K) Matrix vesicle calcification in rat epiphyseal growth plate cartilage prepared anhydrously for electron microscopy 1983, Metabolic Bone Disease and Related Research more D.C. Morris, H.K. Vaananen, H.C. Anderson Matrix vesicle calcification in rat epiphyseal growth plate cartilage pr...
Single-particle electron cryomicroscopy (cryo-EM) involves estimating a set of parameters for each particle image and reconstructing a 3D density map; robust algorithms with accurate parameter estimation are essential for high resolution and automation. We introduce a particle-filter algorithm for cryo-...