7. 小鼠肠道组织 肠道组织里,很多老师都会研究DC细胞,但是DC细胞的标志物CD11C,MHCII在肠道巨噬细胞中也会有表达,老师们在检测和数据分析圈门时要注意哦。 8. 小鼠皮肤组织 除了CD11B,F4/80marker外,可添加EPCAM,CD64,CD24等标...
Depletion of CD11b+Ly6C+MHC Class II+ macrophages accelerates colon inflammation in WT mice induced by DSS treatment.Kenichiro, NishikawaNaohiro, SeoMie, ToriiNei, MaDaisuke, MuraokaIsao, TawaraMasahiro, MasuyaKyosuke, TanakaYoshiyuki, Takei
cells by flow cytometry, we gated for cDCs (CD45+CD11c+MHC-II+CD88–F4/80–Siglec-F–), excluding Siglec-F+alveolar macrophages (AMs), CD88+F4/80+IMs, F4/80+monocytes, and CD88+MHC-II–neutrophils (Supplementary Figs.1dand3a, b). At steady state, there were very few Ly-6C+anti...
Following CD11chighMHCII þ DC subsets were sorted and analyzed in a gene expression microarray: (1) CD103 þ CD11b À , (2) CD103 þ CD11b þ , and (3) CD103 À CD11b þ . (b) Transcript heat map of the B640 genes that are at least twofold differentially expressed ...
Abs used were anti-CD14 (mouse 3C10; ATCC), anti-MHC class I (mouse W6.32; ATCC), and anti-MHC class II (mouse BT-2.9). Saturating concentrations of mAbs recognizing TLR4 (mouse; e-Bioscience) and CD11b (mouse; Santa Cruz Biotechnology) were used to check FG receptor expression ...
TGFβR1 signalling controls dendritic cell homeostasis in the intestine.aExpression of CD103 and CD11b by live CD45+CD11c+MHCII+CD64−DC (left) and relative frequencies of DC subsets (right) in the SILP ofRag1−/−Tgfbr1fl/flmice (Rag1−/−Cre−) orRag1−/−CD11c-Cre....
MHC II 1025 103 104 450 103 104 80 70 60 50 40 30 20 10 0 100 80 70 60 50 40 30 20 10 0 100 260 101 102 103 104 FL2-H 134 101 102 103 FL2-H CD40 104 80 70 60 50 40 30 20 10 0 100 80 70 60 50 40 30 20 10 0 100 62 101 102 103 104 FL2-H 80 70 60 50...