Depletion of CD11b+Ly6C+MHC Class II+ macrophages accelerates colon inflammation in WT mice induced by DSS treatment.Kenichiro, NishikawaNaohiro, SeoMie, ToriiNei, MaDaisuke, MuraokaIsao, TawaraMasahiro, MasuyaKyosuke, TanakaYoshiyuki, Takei
Mechanistically, Rab8a and Rab11 cooperate to induce efficient trafficking of peptide loaded class I MHC molecules from the ER to the cell surface. We propose that targeting Rab8a might serve as a strategy to restore DC functionality in the context of immune aging....
Dynamics of Dendritic Cell Phenotype and Interactions with CD4+ T Cells in Airway Inflammation and Tolerance The DC population derived from tolerized mice was predominantly CD11c+, B220+, Gr-1+, CD11b, and MHC class IIlow, which resembled plasmacytoid-type ... TB Oriss,M Ostroukhova,C Segu...
Following CD11chighMHCII þ DC subsets were sorted and analyzed in a gene expression microarray: (1) CD103 þ CD11b À , (2) CD103 þ CD11b þ , and (3) CD103 À CD11b þ . (b) Transcript heat map of the B640 genes that are at least twofold differentially expressed ...
cells by flow cytometry, we gated for cDCs (CD45+CD11c+MHC-II+CD88–F4/80–Siglec-F–), excluding Siglec-F+alveolar macrophages (AMs), CD88+F4/80+IMs, F4/80+monocytes, and CD88+MHC-II–neutrophils (Supplementary Figs.1dand3a, b). At steady state, there were very few Ly-6C+...
肠道组织里,很多老师都会研究DC细胞,但是DC细胞的标志物CD11C,MHCII在肠道巨噬细胞中也会有表达,老师们在检测和数据分析圈门时要注意哦。 8. 小鼠皮肤组织 除了CD11B,F4/80marker外,可添加EPCAM,CD64,CD24等标志物,用于对巨噬...
肿瘤微环境中,在巨噬细胞与肿瘤细胞相互作用的过程中一方面巨噬细胞使得肿瘤细胞表面的MHC分子标志丢失,得以逃脱免疫系统的攻击;另一方面肿 瘤细胞也使得巨噬细胞的表型发生变化,变成一种促进肿瘤生长的细胞[21]。研究 表明,肿瘤微环境和炎症微环境的根本区别在于肿瘤组织内部存在大量的坏死组 织,因此我们把NTC-Ms作为...
(Fig. 2b). In agreement with previous experiments using BM-derived DCsin vivo6,31or splenic DCsin vitro32, SEA-specific Th2 induction by intestinal lymph DCs required major histocompatibility complex II (MHCII) expression (Fig. 2candSupplementary Fig. 3b), but was independent of their ...
TGFβR1 signalling controls dendritic cell homeostasis in the intestine.aExpression of CD103 and CD11b by live CD45+CD11c+MHCII+CD64−DC (left) and relative frequencies of DC subsets (right) in the SILP ofRag1−/−Tgfbr1fl/flmice (Rag1−/−Cre−) orRag1−/−CD11c-Cre....
The reduced MHC II and costimulatory molecules expressions in F1 mDCs from symptomatic mice, however, did not appear to have significant impact on their capacity to stimulate allogeneic T cell proliferation, at least in in vitro with a DC/T ratio of 1:10 (Figure 2C). Whether a functional ...