METHOD: We incubated whole peripheral blood of AS patients and healthy controls with phosphate-buffered saline (PBS) or lipopolysaccharide (LPS) for 48 h with saturating levels of labeled antibodies (CD66b, CD11b and human leukocyte antigen type DR [HLA-DR]) for flow cytometry. CD11b and HLA...
It has also gained usage as a microglial marker for tissues derived from the nervous system. Research Studies Using CD11b Antibodies in the Tumor Microenvironment Immunoblotting with the CD11b antibody from Wong's lab at Johns Hopkins demonstrates that the hypoxia-inducible factor 1 (HIF-1) ...
an Antibody byInvitrogen Antibodies Applications: IHC-P Reactivity: Mus musculus (House mouse) Images: (16-0041)CD4 Monoclonal Antibody (GK1.5), Functional Grade, eBioscience an Antibody byInvitrogen Antibodies Applications: FC/FACS Reactivity: ...
We then tested whether the CD11b integrin function in CD11b+ leucocytes could be involved in parasite dissemination to the brain by injecting CD11b-binding antibodies in T gondii–parasitized mice. Following relevant antibody injection at day 6 and day 7 after inoculation, the parasite burden decre...
Cells were resuspended at approximately 107/ml FACS buffer and incubated with anti-CD16/32 (2.4G2) for 5 min on ice before staining with specific antibodies. Cells were then washed with 1 ml of FACS buffer, centrifuged, resuspended in 200μl of FACS buffer, and analyzed on a flow ...
age 60.12±7.10)and10 non-COPD patients (age 61.12±7.11) from the medical ward of Dujiangyan republic hospital,.The leukocytes were separated with hydroxyl starch form 2ml peripheral venous blood. The CD 11b antigen of granulopoesis were incubated with fluorescence-labeled CD antibodies. ...
It's well known that antibodies have the intrinsic benefit of high binding affinity and high target selectivity33. As shown in this study, the saturation binding experiment showed that the radiolabeled anti-CD11b bound with high affinity to the Raw-264.7 cells with a half-life: 4.57 ±...
The primary antibodies were used for immunohistochemical (IHC), immuno- fluorescence (IF) analysis and Western blotting (WB) assays: and mouse anti-BrdU antibody (RPN202, diluted at 1:150 for IHC) was obtained from GE Healthcare (Little Chalfond, UK); rabbit anti-CyclinD1 (BM0771, ...
Cells with a similar phenotype are also involved in pro-inflammatory processes, and we therefore wanted to investigate the gene expression and function of these cells in animals that were either subjected to chronic inflammation, or inoculated with tumors. Methods CD11b+Ly6C++ and Ly6G+ cells ...
This study was reviewed and approved by the Ethics Committee of the Southwest Hospital of Army Medical University, and written informed consent for study enrollment and blood collections obtained from all individuals. Antibodies and other reagents Antibodies (Abs) used for flow cytometry were anti-IFN...