尽管免疫功能会随着胃肠道的长度而变化,但尚不清楚不同区域、不同免疫细胞或其他来源的EV在应对肠道屏障挑战方面是否具有不同的作用。众所周知,IEC衍生的囊泡,称为“toleroomes”,显示具有结合抗原的MHCII,并能到达淋巴结,诱导对口服抗原的...
7. 小鼠肠道组织 肠道组织里,很多老师都会研究DC细胞,但是DC细胞的标志物CD11C,MHCII在肠道巨噬细胞中也会有表达,老师们在检测和数据分析圈门时要注意哦。 8. 小鼠皮肤组织 除了CD11B,F4/80marker外,可添加EPCAM,CD64,CD24等标...
CD11c+ MHCIIlo GM-CSF-bone marrow-derived dendritic cells act as antigen donor cells and as antigen presenting cells in neoepitope-elicited tumor immunity against a mouse fibrosarcomaCancer, Immunotherapy, Neoepitopes, Vaccine, Dendritic cells, Adjuvant...
(A) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Anti-Mouse CD11c Antibody, Clone N418, followed by anti-hamster (Armenian) IgG, FITC and anti-mouse MHC class II, APC. (B) Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with a hamster (Armenian) ...
C57BL/6 mouse spleen was digested with collagenase and DNase I. A single cell suspension was prepared to isolate CD11c positive cells using MojoSort™ Mouse CD11c Nanobeads. Cells were stained with CD11c (clone N418) PE and MHC II (clone M5/114.15.2) APC. Dead cells were excluded by 7AA...
Flow cytometric analysis of live mouse bone marrow derived dendritic cells using CD11c (N418) Hamster mAb (PE-Cy7® Conjugate) (right), compared to concentration-matched Armenian Hamster Isotype Control (PE-Cy7® Conjugate) (left), co-stained with MHC Class II (I-A/I-E) (M5/114.15....
Flow cytometric analysis of live mouse bone marrow derived dendritic cells using CD11c (N418) Hamster mAb (APC-Cy7® Conjugate) (right), compared to concentration-matched Armenian Hamster Isotype Control (APC-Cy7® Conjugate) (left), co-stained with MHC Class II (I-A/...
(N418) Mouse IgG2a Recombinant Antibody (CL647-65602, Clone:N418) or CoraLite® Plus 647 Mouse IgG2a Isotype Control (C1.18.4) (CL647-65208, Clone: C1.18.4), Cells were then co-stained with CoraLite® Plus 488 Anti-Mouse MHC Class II (I-A/I-E) (M5/114.15.2) (CL488-65122,...
showed that within the CD11b+MHC-II+CD11c+fractions of the lung and small intestinelamina propria, mutually exclusive expression of the “heat-stable antigen”CD24and CD64 distinguished FLT3-dependent CD24+CD64−DCs from contaminating CSF-1R-dependent CD24−CD64+macrophages (Schlitzer et al....
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