Whole genome sequencing (WGS) approaches can be used to comprehensively explore all types of genomic alterations in cancer and help us to better understand the whole landscape of driver mutations and mutational signatures in cancer genomes and elucidate the functional or clinical implications of these ...
cancer phenotypes to genotypes. Whole genome sequencing is the best approach for discovery science or data mining which can help identify new variants. It also provides a comparison of tumor DNA profiles to that of normal DNA samples to determine novel cancer variants in both coding and non-...
Whole-genome sequencing delivers a comprehensive view, ideal for discovery applications. Newer genome sequencers perform WGS more rapidly than ever.
As whole-genome sequencing for cancer genome analysis becomes a clinical tool, a full understanding of the variables affecting sequencing analysis output is required. Here using tumour-normal sample pairs from two different types of cancer, chronic lymphocytic leukaemia and medulloblastoma, we conduct a...
Whole-genome sequencing (WGS) brings comprehensive insights to cancer genome interpretation. To explore the clinical value of WGS, we sequenced 254 triple-negative breast cancers (TNBCs) for which associated treatment and outcome data were collected between 2010 and 2015 via the population-based Swede...
Whole genome sequencing for childhood cancer in DenmarkRamneek Gupta
Whole genome sequencing (WGS) is becoming the preferred method for molecular genetic diagnosis of rare and unknown diseases and for identification of actionable cancer drivers. Compared to other molecular genetic methods, WGS captures most genomic variat
Deep multi-region whole-genome sequencing reveals heterogeneity and gene-by-environment interactions in treatment-naive, metastatic lung cancer Single-region whole-genome sequencing of from 20 patients showed that tumors in ever-smokers with the strongest tobacco signatures were associated with germline var...
Preparation of sequencing-ready libraries from cfDNA or ctDNA presents a number of technical challenges. Despite cfDNA being present in higher levels in cancer patients than in healthy individuals, the total amount of...
全基因组重测序(Whole Genome Sequencing, WGS):通过对全基因组DNA进行测序,可获取最全的基因组核酸变异信息,从而研究其与疾病和其它生物性状相关性。全基因组重测序可获取最全的基因组信息。 技术流程 技术参数 测序平台与方式:HiSeq, PE150 测序深度:30X,50X或更高(90G, 150G或更高的数据量) ...