Whole-genome sequencing delivers a comprehensive view, ideal for discovery applications. Newer genome sequencers perform WGS more rapidly than ever.
Whole genome sequencing (WGS) is the comprehensive read and analysis of an entire genome, including non-coding regions of the genome.
Whole-genome sequencing of paired tumor/normal was performed in all cases. In short, raw sequencing data were processed using bcl2fastq (versions 2.17 to 2.20), mapped to the human reference genome GRCh37 using BWA-mem v0.7.Sa and GATK BQSR and Haplotype Caller v3.4.46 and Strelka v1.0.1...
et al. PhyResSE: a web tool delineating Mycobacterium tuberculosis antibiotic resistance and lineage from whole-genome sequencing data. J. Clin. Microbiol. 53, 1908–1914 (2015). CAS PubMed PubMed Central Google Scholar Bradley, P. et al. Rapid antibiotic-resistance predictions from genome ...
The whole-genome resequencing data was generated for the samples from 36 wild mice by Illumina technology. Approximately 1.4 Tb data was acquired. Raw sequencing data for the 36 wild mice ranged from 24.4 to 54.3 Gb (Additional file1: Table S3). After mapping to the mouse reference genome (...
Whole genome sequencing (WGS) offers an alternative data source to allow for the detection and characterization of the copy number across different genomic regions in a single experiment. A plethora of tools have been developed to utilize WGS data for CNV detection. None of these tools are ...
target. Assembly of whole genome shotgun sequencingdatawas difficult and required sophisticated computer programs and powerful supercomputers, and, even in the years following the completion of the HGP, whole genome shotgun sequence assembly remained a significant challenge for whole genome sequencing ...
Numerous viral sequences have been reported in the whole-genome sequencing (WGS) data of human blood. However, it is not clear to what degree the virus-mappable reads represent true viral sequences rather than random-mapping or noise originating from sample preparation, sequencing processes, or ...
Whole genome sequencing (WGS) is becoming the preferred method for molecular genetic diagnosis of rare and unknown diseases and for identification of actionable cancer drivers. Compared to other molecular genetic methods, WGS captures most genomic variat
indicating that the qualities of the whole genome sequencing data were found to be sufficiently good for downstream analyses. The average sequencing coverage of the 58 genomes was 11.4 x, varying from 8.1 to 15.8 × coverage (Supplementary Data2). Average depth was lower, with slight varia...