Whole-genome sequencing delivers a comprehensive view, ideal for discovery applications. Newer genome sequencers perform WGS more rapidly than ever.
Whole genome sequencing methods2488,798 N/S Polyphen: probablydamaging (0.998) SIFT: deleterious (0)Middletonusing the Covaris SHopkintoncollecting three
For WES, samples can be prepared using multiple library preparation methods. The xGen whole exome sequencing solution employs thexGen DNA Library Prep Kit EZ, which uses enzymatic fragmentation to shear high-quality DNA samples. This kit uses a TA ligation-based library prep and includes an enzym...
sequencing methods. Next-generation sequencing approaches are characterized generally by the massively parallel production of short sequences, in which multiple DNA fragments are generated simultaneously and in sufficient quantity to redundantly represent every base in the target genome. Although such ...
Whole genome sequencing (WGS) is becoming the preferred method for molecular genetic diagnosis of rare and unknown diseases and for identification of actionable cancer drivers. Compared to other molecular genetic methods, WGS captures most genomic variat
Methods We examined the feasibility of using whole-genome sequencing (WGS) data to design tumour-specific polymerase chain reaction (PCR)-based MRD tests (WGS-MRD) in 18 children with high-risk relapsed cancer, including ALL, high-risk neuroblastoma (HR-NB) and Ewing sarcoma (EWS) (n =...
Accurate whole-genome sequencing-based epidemiological surveillance of Mycobacterium tuberculosis. Methods Microbiol. 42, 359–394 (2015). CAS Google Scholar Simonyan, V., Goecks, J. & Mazumder, R. Biocompute objects — a step towards evaluation and validation of biomedical scientific computations....
Methods Ethics Keeping and management of wild and laboratory mice included in this study followed the guidelines of Institute of Zoology and were approved by the Ethics Committee of the Institute of Zoology (IOZ20190048). Samples and sequencing ...
Objectives: The objectives of this study were to identify antimicrobial resistance genotypes for Campylobacter and to evaluate the correlation between resistance phenotypes and genotypes using in vitro antimicrobial susceptibility testing and whole-genome sequencing (WGS). Methods: One hundred-fourteen Campylob...
Identification methods for microbiologists This second edition retains most of the sections of the first [Abstr. Hyg. 1969, 44, abstr. 1344] and these have been revised, sometimes with changed autho... MB Gibbs,AD Shapton 被引量: 305发表: 1980年 Substructural and progressive structural ...