Watch one of our videos or check out one of our written tutorials to learn more about whole genome sequence analysis and whole exome sequencing analysis.Exome Analysis Tutorial See how to align exome resequencing data from all major NGS platforms against a reference sequence with unsurpassed ease...
Whole Genome Sequencing AnalysisRui YinChee Keong KwohJie ZhengEncyclopedia of Bioinformatics and Computational Biology
Objectives: The objectives of this study were to identify antimicrobial resistance genotypes for Campylobacter and to evaluate the correlation between resistance phenotypes and genotypes using in vitro antimicrobial susceptibility testing and whole-genome sequencing (WGS). Methods: One hundred-fourteen Campylob...
-genome sequencing, and genotypes have been called for each individual for each site in the genome using a standard pipeline. The data has been filtered to retain onlybi-allelicsingle nucleotide polymorphisms (SNPs), and these have been furtherthinnedto reduce the file size for this tutorial....
Whole-genome sequencing is suitable for the study of disease-specific genomic abnormalities from cfDNA. TheInvitrogen Collibri PS DNA Library Prep Kit for Illumina Systemsenables consistent and reproducible cfDNA library prepa...
Exome sequencing is a targeted sequencing approach that is restricted to the protein-coding regions of genomes. The exome is estimated to encompass approximately 1% of the genome, yet contains approximately 85% of disease-causing mutations [1]. For genetic re...
Whole genome sequencing (WGS) improves Mendelian disorder diagnosis over whole exome sequencing (WES); however, additional diagnostic yields and costs remain undefined. We investigated differences between diagnostic and cost outcomes of WGS and WES in a cohort with suspected Mendelian disorders. WGS was...
Genome annotation Multi-Locus Sequence Typing (MLST, optional) Antibiotic resistance genes screening Virulence genes screening Pangenome comparison (optional) When working withEscherichia,KlebsiellaorSalmonellasequencing data, extensive analysis can be enabled (by using the-g/--generaoption), including: ...
The tutorial for performing single-/multi-trait association analysis of whole-genome/whole-exome sequencing (WGS/WES) studies using FAVORannotator, STAARpipeline and STAARpipelineSummary - xihaoli/STAARpipeline-Tutorial
(Supplementary Table9). PCR duplicates were removed using the MarkDuplicates tools from the Picard suite. Nuclear and mitochondrial genome consensus sequences were called using mpileup in SAMtools. SNPs were called using HaplotypeCaller in the Genomic Analysis Toolkit85(GATK) for all stoats and again ...